Barhoine Mariama, Moustaoui Fatima, Hammani Omayma, Aghrouch Mohamed, Lemkhente Zohra, Belhabib Zineb, Bajaddoub Zineb, Touyar Anass, Aqoudad Nourdin, Rherissi Bouchra, El Kadmiri Nadia, Idaghdour Youssef, Boubrik Fatima, Belmouden Ahmed
Cell Biology and Molecular Genetics Laboratory, Faculty of Sciences, Ibnou Zohr University, Agadir 80000, Morocco.
Medical Analysis Laboratory, Regional Hospital Centre Hassan II, Agadir 80000, Morocco.
Pathogens. 2025 Mar 14;14(3):279. doi: 10.3390/pathogens14030279.
() possess an arsenal of virulence genes that makes them the main etiological factor in gastric diseases. In this study, 120 southern Moroccan patients who were dyspeptic were profiled to investigate the potential association between disease severity and the combination of multiple virulence genes. Gastric biopsies were taken from patients, followed by histopathological evaluation and genotyping of using PCR. was detected in 58.3% of cases, and genotypes were distributed as follows: (94.3%), (62.9%), (60%), (55.7%), (54.3%), (51.4%), (31.4%), (45.7%), (47.1%), (30%), and (7.1%). Statistically significant associations with males were observed for the , , and genes and multiple strain infections. Multivariate analysis revealed an association between and heightened neutrophil activity, with an odds ratio (OR) of 4.99 ( = 0.03). The gene combination [ (+), (+), (+), , and (+)] emerged as a predictive factor for gastric cancer (OR = 11.10, = 0.046), while the combination [ (-), (-), (-), , (+)] was associated with gastric atrophy (OR = 10.25, = 0.016). Age (≤40 years) (OR = 5.87, = 0.013) and moderate to severe bacterial density (OR = 15.38, = 0.017) were identified as predictive factors for follicular gastritis. These findings underscore the significance of multigene profiling as a prognostic marker and emphasize the importance of age and sex in preventing adverse outcomes in severe gastric diseases.
（）拥有一系列毒力基因，使其成为胃部疾病的主要病因。在本研究中，对120名摩洛哥南部消化不良患者进行分析，以调查疾病严重程度与多种毒力基因组合之间的潜在关联。从患者身上采集胃活检组织，随后进行组织病理学评估，并通过聚合酶链反应（PCR）对（原文此处缺失具体病原体名称）进行基因分型。在58.3%的病例中检测到（原文此处缺失具体病原体名称），其基因型分布如下：（94.3%），（62.9%），（60%），（55.7%），（54.3%），（51.4%），（31.4%），（45.7%），（47.1%），（30%），以及（7.1%）。观察到、和基因以及多种菌株感染与男性存在统计学显著关联。多变量分析显示与中性粒细胞活性增强之间存在关联，优势比（OR）为4.99（=0.03）。基因组合[（+），（+），（+），以及（+）]成为胃癌的预测因素（OR = 11.10，= 0.046），而组合[（-），（-），（-），，（+）]与胃萎缩相关（OR = 10.25，= 0.016）。年龄（≤40岁）（OR = 5.87，= 0.013）和中度至重度细菌密度（OR = 15.38，= 0.017）被确定为滤泡性胃炎的预测因素。这些发现强调了多基因分析作为预后标志物的重要性，并强调了年龄和性别在预防严重胃部疾病不良结局中的重要性。
