Denchev V, Mitov I, Marinova S, Linde K
Research Institute of Infectious and Parasitic Diseases, Medical Academy, Sofia, Bulgaria.
J Hyg Epidemiol Microbiol Immunol. 1988;32(4):457-65.
Local and systemic immune response was studied in 3 groups of rabbits immunized and reimmunized 190 days later with S. typhimurium double-marker attenuated strain 1,771 and doses ranged from 20.10(9) to 0.2.10(9) cells. Another group of rabbits was immunized with extract (hydroxylammine) vaccine. It was found that the attenuated strain persisted for considerable time in the gut, and induced pronounced and continuous immune response as measured by the passive hemagglutination test. The serum antibody response had the character of a secondary one with switching on the synthesis of IgM to IgG already after basic immunisation. By the Coombs' technique it was shown that specific immunoglobulins demonstrated in feces were secretory antibodies of the class IgA (SIgA). The immune response developed after reimmunization was still more vigorous and prolonged pointing to an immune memory existance. It was possible to obtain well manifested immunity with the lowest dose. The extract vaccine revealed only weak and transitory serum and intestinal antibody levels without SIgA appearance. The results obtained in this setup make S. typhimurium 1771 a perspective candidate for a live vaccine.
对3组家兔进行了局部和全身免疫反应的研究，这些家兔用鼠伤寒沙门氏菌双标记减毒株1771免疫，并在190天后再次免疫，剂量范围为20×10⁹至0.2×10⁹个细胞。另一组家兔用提取物（羟胺）疫苗免疫。结果发现，减毒株在肠道中持续存在相当长的时间，并通过被动血凝试验检测到诱导了明显且持续的免疫反应。血清抗体反应具有二次反应的特征，在基础免疫后即开始从IgM合成转换为IgG合成。通过库姆斯技术表明，粪便中显示的特异性免疫球蛋白是IgA类分泌型抗体（SIgA）。再次免疫后产生的免疫反应更加剧烈和持久，表明存在免疫记忆。用最低剂量就可以获得明显的免疫力。提取物疫苗仅显示出微弱且短暂的血清和肠道抗体水平，且未出现SIgA。在此实验设置中获得的结果使鼠伤寒沙门氏菌1771成为一种有前景的活疫苗候选株。
