白色念珠菌细胞壁中几丁质与葡聚糖之间糖苷键的证据。

Evidence for a glycosidic linkage between chitin and glucan in the cell wall of Candida albicans.

作者信息

Surarit R, Gopal P K, Shepherd M G

机构信息

Experimental Oral Biology Unit, Faculty of Dentistry, University of Otago, Dunedin, New Zealand.

出版信息

J Gen Microbiol. 1988 Jun;134(6):1723-30. doi: 10.1099/00221287-134-6-1723.

DOI:10.1099/00221287-134-6-1723

PMID:3065455

Abstract

The alkali-insoluble glucan was isolated from regenerating spheroplasts and intact cells of Candida albicans. Sequential enzymic hydrolysis of this fraction by Zymolyase 100T and purified chitinase and subsequent gel filtration produced a fraction which was enriched in glycosaminoglycans. This fraction was analysed by partial acid hydrolysis, TLC and GLC-MS. The GLC-MS peaks identified included 2,3,4,6-tetra-O-methylglucitol acetate and 2,3,4-tri-O-methylglucitol acetate of beta-1,6-glucan and the 3,6-di-O-methyl-2-N-methylglucosaminitol acetate of chitin. In addition, 3-O-methyl-2-N-methylglucosaminitol acetate was identified, which indicated a branch point in chitin. These data provide evidence for a covalent linkage between chitin and beta-(1,6)-glucan through a glycosidic linkage at position 6 of N-acetylglucosamine and position 1 of the glucose in the glucan.

摘要

从白色念珠菌的再生原生质体和完整细胞中分离出碱不溶性葡聚糖。用溶菌酶100T和纯化的几丁质酶对该组分进行顺序酶解，随后进行凝胶过滤，得到富含糖胺聚糖的组分。该组分通过部分酸水解、薄层层析和气相色谱-质谱联用进行分析。气相色谱-质谱联用鉴定出的峰包括β-1,6-葡聚糖的2,3,4,6-四-O-甲基葡糖醇乙酸酯和2,3,4-三-O-甲基葡糖醇乙酸酯，以及几丁质的3,6-二-O-甲基-2-N-甲基葡糖胺醇乙酸酯。此外，还鉴定出3-O-甲基-2-N-甲基葡糖胺醇乙酸酯，这表明几丁质中存在一个分支点。这些数据为几丁质和β-(1,6)-葡聚糖之间通过N-乙酰葡糖胺第6位的糖苷键和葡聚糖中葡萄糖第1位的糖苷键形成共价连接提供了证据。