Elorza M V, Rico H, Gozalbo D, Sentandreu R
Antonie Van Leeuwenhoek. 1983 Nov;49(4-5):457-69. doi: 10.1007/BF00399324.
The transition of blastospores to the mycelial phase in Candida albicans was induced after the blastospores were kept at 4 degrees C for several hours and then transferred to a fresh medium prewarmed at 37 degrees C. Glucan was the most abundant polymer in the wall in the two morphogenetic forms but the amount of chitin was higher in the mycelial form than in blastospores. Efficient protoplasting required reducing agents and proteases together with beta-glucanases (zymolyase). Protein synthesis in regenerating protoplasts was initiated after about 30 min. Chitin synthetase, initially very low, was incorporated in important amounts into cell membranes mainly in a zymogenic state. After a few hours chitin was the most abundant polymer found in the aberrant wall of the regenerating protoplast.
白色念珠菌的芽生孢子在4℃保存数小时后，转移至预热至37℃的新鲜培养基中，可诱导其向菌丝体阶段转变。葡聚糖是两种形态发生形式细胞壁中最丰富的聚合物，但菌丝体形式中几丁质的含量高于芽生孢子。高效原生质体制备需要还原剂、蛋白酶以及β-葡聚糖酶（溶壁酶）。再生原生质体中的蛋白质合成在约30分钟后开始。几丁质合成酶最初含量很低，主要以酶原状态大量整合到细胞膜中。数小时后，几丁质是在再生原生质体异常细胞壁中发现的最丰富的聚合物。
