Department of Fish Diseases, National Veterinary Research Institute, 57 Partyzantow Avenue, 24-100, Pulawy, Poland.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.
BMC Genomics. 2019 Jan 16;20(1):46. doi: 10.1186/s12864-018-5266-9.
The mechanism of latency and the ability of the cyprinid herpesvirus 3 (CyHV-3) to establish life-long infections in carp remains poorly understood. To explain the role of miRNAs in this process we applied a range of molecular tools including high-throughput sequencing of RNA libraries constructed from the blood samples of infected fish followed by bioinformatic and functional analyses which show that CyHV-3 profoundly influences the expression of host miRNAs in vivo.
We demonstrated the changed expression of 27 miRNAs in the clinical phase and 5 in the latent phase of infection. We also identified 23 novel, not previously reported sequences, from which 8 showed altered expressions in control phase, 10 in clinical phase and 5 in latent phase of infection.
The results of our analysis expand the knowledge of common carp microRNAs engaged during CyHV-3 infection and provide a useful basis for the further study of the mechanism of CyHV-3 induced pathology.
鲤鱼疱疹病毒 3（CyHV-3）潜伏的机制及其在鲤鱼中建立终身感染的能力仍未得到充分理解。为了解释 miRNA 在这一过程中的作用，我们应用了一系列分子工具，包括对感染鱼血液样本构建的 RNA 文库进行高通量测序，然后进行生物信息学和功能分析，结果表明 CyHV-3 会深刻影响宿主 miRNA 的体内表达。
我们在临床阶段证明了 27 个 miRNA 的表达发生了变化，在潜伏阶段则有 5 个 miRNA 的表达发生了变化。我们还从之前未报道过的 23 个新序列中鉴定出了 8 个在对照阶段表达发生改变，10 个在临床阶段，5 个在潜伏阶段。
我们的分析结果扩展了鲤鱼参与 CyHV-3 感染的 microRNAs 的知识，并为进一步研究 CyHV-3 诱导的病理学机制提供了有用的基础。
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