Wollenberg Rasmus Dam, Sondergaard Teis Esben, Nielsen Mikkel Rank, Knutsson Simon, Pedersen Tobias Bruun, Westphal Klaus Ringsborg, Wimmer Reinhard, Gardiner Donald Max, Sørensen Jens Laurids
Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Department of Chemistry and Bioscience, Aalborg University, Esbjerg, Denmark.
Fungal Biol. 2019 Jan;123(1):10-17. doi: 10.1016/j.funbio.2018.10.004. Epub 2018 Oct 28.
Fusarium pseudograminearum is a significant pathogen of cereals in arid regions worldwide and has the ability to produce numerous bioactive secondary metabolites. The genome sequences of seven F. pseudograminearum strains have been published and in one of these strains, C5834, we identified an intact gene cluster responsible for biosynthesis of the cyclic lipopeptide fusaristatin A. The high level of sequence identity of the fusaristatin cluster remnant in strains that do not produce fusaristatin suggests that the absence of the cluster evolved once, and subsequently the resulting locus with the cluster fragments became widely dispersed among strains of F. pseudograminearum in Australia. We examined a selection of 99 Australian F. pseudograminearum isolates to determine how widespread the ability to produce fusaristatin A is in F. pseudograminearum. We identified 15 fusaristatin producing strains, all originating from Western Australia. Phylogenetic analyses could not support a division of F. pseudograminearum into fusaristatin producing and nonproducing populations, which could indicate the loss has occurred relatively recent.
拟禾本科镰孢菌是全球干旱地区谷物的重要病原菌，能够产生多种具有生物活性的次生代谢产物。已公布了7株拟禾本科镰孢菌的基因组序列，在其中一株C5834中，我们鉴定出一个完整的基因簇，该基因簇负责环状脂肽镰刀菌素A的生物合成。在不产生镰刀菌素的菌株中，镰刀菌素基因簇残余序列具有高度的序列同一性，这表明该基因簇的缺失仅发生过一次，随后带有基因簇片段的位点在澳大利亚的拟禾本科镰孢菌菌株中广泛分布。我们检测了99株澳大利亚拟禾本科镰孢菌分离株，以确定产生镰刀菌素A的能力在拟禾本科镰孢菌中的分布情况。我们鉴定出15株产生镰刀菌素的菌株，均来自西澳大利亚。系统发育分析不支持将拟禾本科镰孢菌分为产生镰刀菌素和不产生镰刀菌素的群体，这可能表明这种缺失是相对近期发生的。
