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FOXN2在乳腺癌中表达下调，并通过调控SLUG来调节迁移、侵袭和上皮-间质转化。

FOXN2 is downregulated in breast cancer and regulates migration, invasion, and epithelial- mesenchymal transition through regulation of SLUG.

作者信息

Ye Hui, Duan Meiling

机构信息

Department of Galactophore, Linyi Central Hospital of Shandong, Linyi, 276400, China.

Department of Respiratory One, Linyi Central Hospital of Shandong, Linyi, 276400, China,

出版信息

Cancer Manag Res. 2019 Jan 4;11:525-535. doi: 10.2147/CMAR.S176938. eCollection 2019.

DOI:10.2147/CMAR.S176938
PMID:30655703
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6324606/
Abstract

INTRODUCTION

Forkhead box (FOX) N2 (FOXN2), a member of FOX protein family, has been reported to play critical roles in some types of cancers. However, the expression and function of FOXN2 in breast cancer remain unclear.

METHODS

In the present work, we explored the detailed molecular mechanism of FOXN2 in breast cancer. We performed RT-qPCR and Western blotting analysis to detect the expression of FOXN2 in breast cancer. Colony formation assay, CCK-8 assay, wound healing assay, and Transwell assay were used to determine the effect of FOXN2 on cell proliferation, migration, and invasion in breast cancer.

RESULTS

Our results demonstrated that FOXN2 was downregulated in breast cancer tissues and cell lines. Downregulation of FOXN2 was correlated with tumor size, pathological grade, and lymph node metastasis. The in vitro experiments revealed that the ectopic expression of FOXN2 significantly suppressed the proliferation, migration, and invasiveness of breast cancer cells, and inhibition of FOXN2 promoted the proliferation, migration, and invasiveness of breast cancer cells. Moreover, inhibition of FOXN2 facilitated epithelial-mesenchymal transition (EMT) through regulation of SLUG.

CONCLUSION

Taken together, our results showed for the first time that FOXN2 plays an essential role in cell proliferation and invasion. Thus, FOXN2 may be an attractive therapeutic target for the treatment of breast cancer.

摘要

引言

叉头框（FOX）N2（FOXN2）是FOX蛋白家族的成员，据报道在某些类型的癌症中发挥关键作用。然而，FOXN2在乳腺癌中的表达和功能仍不清楚。

方法

在本研究中，我们探讨了FOXN2在乳腺癌中的详细分子机制。我们进行了RT-qPCR和蛋白质免疫印迹分析以检测FOXN2在乳腺癌中的表达。采用集落形成试验、CCK-8试验、伤口愈合试验和Transwell试验来确定FOXN2对乳腺癌细胞增殖、迁移和侵袭的影响。

结果

我们的结果表明，FOXN2在乳腺癌组织和细胞系中表达下调。FOXN2的下调与肿瘤大小、病理分级和淋巴结转移相关。体外实验表明，FOXN2的异位表达显著抑制乳腺癌细胞的增殖、迁移和侵袭能力，而抑制FOXN2则促进乳腺癌细胞的增殖、迁移和侵袭能力。此外，抑制FOXN2通过调节SLUG促进上皮-间质转化（EMT）。

结论

综上所述，我们的结果首次表明FOXN2在细胞增殖和侵袭中起重要作用。因此，FOXN2可能是治疗乳腺癌的一个有吸引力的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/5d72116d955f/cmar-11-525Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/50224a78edc3/cmar-11-525Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/df316f02e0e0/cmar-11-525Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/746cc4cbc04f/cmar-11-525Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/7fe54c02976e/cmar-11-525Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/11fb10fefd4f/cmar-11-525Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/5d72116d955f/cmar-11-525Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/50224a78edc3/cmar-11-525Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/df316f02e0e0/cmar-11-525Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/746cc4cbc04f/cmar-11-525Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/7fe54c02976e/cmar-11-525Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/11fb10fefd4f/cmar-11-525Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/01bc/6324606/5d72116d955f/cmar-11-525Fig6.jpg

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本文引用的文献

1
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2
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Cell Death Differ. 2018 Aug;25(8):1473-1485. doi: 10.1038/s41418-017-0055-6. Epub 2018 Feb 2.
3
PTBP3-Mediated Regulation of ZEB1 mRNA Stability Promotes Epithelial-Mesenchymal Transition in Breast Cancer.
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Hum Cell. 2024 Sep;37(5):1475-1488. doi: 10.1007/s13577-024-01091-w. Epub 2024 Jun 16.
4
A Mouse-Specific Model to Detect Genes under Selection in Tumors.一种用于检测肿瘤中受选择基因的小鼠特异性模型。
Cancers (Basel). 2023 Oct 26;15(21):5156. doi: 10.3390/cancers15215156.
5
Protein Z modulates the metastasis of lung adenocarcinoma cells.蛋白Z调节肺腺癌细胞的转移。
Open Life Sci. 2023 Jul 28;18(1):20220667. doi: 10.1515/biol-2022-0667. eCollection 2023.
6
Hsa_circ_0001741 Suppresses Ovarian Cancer Cell Proliferations Through Adsorption of miR-188-5p and Promotion of FOXN2 Expression.Hsa_circ_0001741 通过吸附 miR-188-5p 和促进 FOXN2 表达来抑制卵巢癌细胞增殖。
Mol Biotechnol. 2024 Jun;66(6):1477-1483. doi: 10.1007/s12033-023-00773-4. Epub 2023 Jun 15.
7
MicroRNA-188-5p inhibits hepatocellular carcinoma proliferation and migration by targeting forkhead box N2.微小 RNA-188-5p 通过靶向叉头框 N2 抑制肝癌增殖和迁移。
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8
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9
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Front Cell Dev Biol. 2022 May 4;10:858336. doi: 10.3389/fcell.2022.858336. eCollection 2022.
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Cancer Res. 2018 Jan 15;78(2):387-398. doi: 10.1158/0008-5472.CAN-17-0883. Epub 2017 Nov 29.
4
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5
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6
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7
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8
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9
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Cell Biochem Biophys. 2015 Jun;72(2):333-8. doi: 10.1007/s12013-014-0459-6.
10
Signaling mechanisms of the epithelial-mesenchymal transition.上皮-间质转化的信号传导机制
Sci Signal. 2014 Sep 23;7(344):re8. doi: 10.1126/scisignal.2005189.