Peng Jin, Yang Kai-Ying, Li Huan, Zheng Shan-Shan, Pan Xue-Yi
Department of Hematology, The First Affiliated Hospital of Guangdong Pharmaceutical University, 19 Nong Lin Road, Yuexiu District, Guangzhou 510080 Guangdong, China.
Open Life Sci. 2023 Jul 28;18(1):20220667. doi: 10.1515/biol-2022-0667. eCollection 2023.
Protein Z (PZ), a vitamin-K-dependent anticoagulant glycoprotein, is reported to be highly expressed in various malignant tissues and correlated with a poor prognosis in patients with lung cancer. This study aimed to investigate the pathological activity of PZ on lung cancer cell migration, invasion, and metastasis. PZ was assessed by Western blot in three non-small-cell lung cancer cell lines (A549, H1299, and H1975). Meanwhile,western blot was used to detect the expression of EMT pathway-related proteins (Slug, Vimentin, and N-cadherin) in the A549 cells knocked down with siRNA. The cellular proliferation, migration, and invasion were detected by Cell Counting Kit (CCK)-8, wound healing, and Transwell assays in the A549 cells. The results showed that PZ expression was higher in A549, H1299, and H1975 cells, according to Western blot. CCK-8, wound healing, and Transwell assays showed that knockdown of PZ significantly decreased cellular proliferation, migration, and invasion, as well as the protein levels of Slug, Vimentin, and N-cadherin in the A549 cells. In conclusion, the pro-metastasis activity of PZ may modulate the epithelial-mesenchymal transition pathway in lung cancer A549 cells.
蛋白Z（PZ）是一种维生素K依赖的抗凝糖蛋白，据报道在各种恶性组织中高表达，且与肺癌患者的不良预后相关。本研究旨在探讨PZ对肺癌细胞迁移、侵袭和转移的病理活性。通过蛋白质印迹法对三种非小细胞肺癌细胞系（A549、H1299和H1975）中的PZ进行评估。同时，用蛋白质印迹法检测经小干扰RNA（siRNA）敲低的A549细胞中上皮-间质转化（EMT）途径相关蛋白（Slug、波形蛋白和N-钙黏蛋白）的表达。通过细胞计数试剂盒（CCK）-8、伤口愈合实验和Transwell实验检测A549细胞的增殖、迁移和侵袭能力。蛋白质印迹结果显示，A549、H1299和H1975细胞中PZ表达较高。CCK-8、伤口愈合实验和Transwell实验表明，敲低PZ可显著降低A549细胞的增殖、迁移和侵袭能力，以及Slug、波形蛋白和N-钙黏蛋白的蛋白水平。总之，PZ的促转移活性可能通过调节肺癌A549细胞中的上皮-间质转化途径发挥作用。
