Qiu Jing, Zhao Juan, Zuo Anjun, Liu Lan, Liu Qiaoqiao, Pan Huazheng, Yuan Xiao
Department of Stomatology, Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China.
Department of Pediatrics, Jiaozhou People's Hospital, Qingdao, Shandong 266300, P.R. China.
Oncol Lett. 2019 Jan;17(1):525-531. doi: 10.3892/ol.2018.9536. Epub 2018 Oct 2.
Oral squamous cell carcinoma (OSCC) is one of the most fatal types of oral cancer worldwide. Forkhead box M1 (FOXM1) is associated with the occurrence and development of a number of types of human cancer, but its function in OSCC remains unclear. The present study aimed to explore the effect of FOXM1 downregulation using lentivirus-mediated short hairpin (sh)RNA against FOXM1 (LV-shFOXM1) in the cell line Tca8113 . Infection of Tca8113 cells with LV-shFOXM1 inhibited the mRNA and protein expression level of FOXM1. The downregulation of FOXM1 resulted in cell cycle arrest of Tca8113 cells, and the inhibition of proliferation, migration and invasion. The protein expression level of cyclins B1 and D1 were downregulated, whereas those of p27 and p21 were upregulated following infection with LV-shFOXM1, compared with the blank control and LV-shCON groups. In addition, FOXM1 downregulation decreased the expression of matrix metalloproteinase-2 and LV-shFOXM1 significantly suppressed OSCC cell viability. Therefore, FOXM1 may be a target for the treatment of OSCC.
口腔鳞状细胞癌（OSCC）是全球最致命的口腔癌类型之一。叉头框M1（FOXM1）与多种人类癌症的发生和发展相关，但其在OSCC中的作用仍不清楚。本研究旨在探讨使用慢病毒介导的针对FOXM1的短发夹（sh）RNA（LV-shFOXM1）下调FOXM1在Tca8113细胞系中的作用。用LV-shFOXM1感染Tca8113细胞可抑制FOXM1的mRNA和蛋白表达水平。FOXM1的下调导致Tca8113细胞的细胞周期停滞，并抑制其增殖、迁移和侵袭。与空白对照组和LV-shCON组相比，用LV-shFOXM1感染后，细胞周期蛋白B1和D1的蛋白表达水平下调，而p27和p21的表达上调。此外，FOXM1的下调降低了基质金属蛋白酶-2的表达，LV-shFOXM1显著抑制了OSCC细胞的活力。因此，FOXM1可能是治疗OSCC的一个靶点。
