Gao Pengjie, Fan Rui, Ge Tao
Department of Stomatology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China.
Oncol Lett. 2019 Jan;17(1):951-957. doi: 10.3892/ol.2018.9709. Epub 2018 Nov 15.
Accumulating evidence indicates that long non-coding RNAs (lncRNAs) serve important roles in various tumor types, including colorectal cancer and gastric cancer. The present study aimed to investigate the contribution of the lncRNA small nucleolar RNA host gene 20 (SNHG20) in oral squamous cell carcinoma (OSCC) progression. It was demonstrated that SNHG20 expression was significantly increased in OSCC tissue specimens, compared with in adjacent non-tumor tissue specimens. The increased SNHG20 expression in OSCC tissue specimens was associated with tumor differentiation and Tumor-Node-Metastasis stage. Kaplan-Meier analysis and log-rank tests indicated that Higher SNHG20 expression predicted a poor overall survival (OS) rate in patients with OSCC. Multivariate Cox proportional hazards regression analysis demonstrated that increased SNHG20 expression was an independent predictor for the OS of patients with OSCC. Knockdown of SNHG20 expression in OSCC cells suppressed proliferation. The cell proliferation-associated proteins proliferating cell nuclear antigen and Ki67 expression levels were reduced when SNHG20 was knocked down in OSCC cells; thus, the results indicated that SHNG20 may serve as a predictor and potential target for OSCC treatment.
越来越多的证据表明，长链非编码RNA（lncRNA）在包括结直肠癌和胃癌在内的多种肿瘤类型中发挥着重要作用。本研究旨在探讨长链非编码RNA小核仁RNA宿主基因20（SNHG20）在口腔鳞状细胞癌（OSCC）进展中的作用。结果表明，与相邻的非肿瘤组织标本相比，OSCC组织标本中SNHG20的表达显著增加。OSCC组织标本中SNHG20表达的增加与肿瘤分化和肿瘤-淋巴结-转移分期相关。Kaplan-Meier分析和对数秩检验表明，较高的SNHG20表达预示着OSCC患者的总生存率（OS）较差。多变量Cox比例风险回归分析表明，SNHG20表达增加是OSCC患者OS的独立预测因素。敲低OSCC细胞中SNHG20的表达可抑制细胞增殖。当在OSCC细胞中敲低SNHG20时，细胞增殖相关蛋白增殖细胞核抗原和Ki67的表达水平降低；因此，结果表明SHNG20可能作为OSCC治疗的预测指标和潜在靶点。
