Zhou Xiaoli, Jia Lizhou, Sun Yangyang, Xu Lingyun, Wang Xudong, Tang Qi
Department of Pathology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu 213003, P.R. China.
Department of Pathology, Nanjing Medical University, Nanjing, Jiangsu 211166, P.R. China.
Oncol Lett. 2019 Jan;17(1):1146-1152. doi: 10.3892/ol.2018.9729. Epub 2018 Nov 19.
Sperm-associated antigen 5 (SPAG5) is currently considered to serve a role in promoting tumor cell growth and is overexpressed in several types of human cancer. However, to the best of our knowledge, the association of SPAG5 with molecular subtypes of patients with breast cancer (BC) remains to be fully investigated. Reverse transcription-quantitative polymerase chain reaction and immunohistochemistry on tissue microarrays were used in the current study to detect the expression levels of SPAG5 mRNA and protein, respectively, in BC. The association between SPAG5 mRNA and protein levels, and clinical characteristics and prognostic information were investigated. SPAG5 mRNA and protein levels were identified to be higher in BC tissues compared with matched adjacent nontumor tissues. High expression level of SPAG5 protein was associated with tumor size, histological grade, estrogen receptor expression, Ki-67 expression, lymph node status, tumor-node-metastasis (TNM) stage and the triple-negative BC subtype. In addition, high expression level of SPAG5 protein was associated with a poor prognosis in patients with BC. In summary, the current study suggests that SPAG5 is a novel and useful prognostic biomarker in BC.
精子相关抗原5（SPAG5）目前被认为在促进肿瘤细胞生长中发挥作用，且在多种人类癌症中过表达。然而，据我们所知，SPAG5与乳腺癌（BC）患者分子亚型之间的关联仍有待充分研究。本研究采用逆转录定量聚合酶链反应和组织芯片免疫组化分别检测BC中SPAG5 mRNA和蛋白的表达水平。研究了SPAG5 mRNA和蛋白水平与临床特征及预后信息之间的关联。与匹配的相邻非肿瘤组织相比，BC组织中SPAG5 mRNA和蛋白水平更高。SPAG5蛋白高表达水平与肿瘤大小、组织学分级、雌激素受体表达、Ki-67表达、淋巴结状态、肿瘤-淋巴结-转移（TNM）分期及三阴性BC亚型相关。此外，SPAG5蛋白高表达水平与BC患者预后不良相关。总之，本研究表明SPAG5是BC中一种新型且有用的预后生物标志物。
