Department of Pathology, Pukou District Central Hospital, Pukou Branch of Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, 166 Shanghe street, Nanjing, Jiangsu, China.
Department of Neurology, Affiliated Drum Tower Hospital of Nanjing University Medical School, 321 ZhongShan Road, Nanjing, Jiangsu, China.
Biosci Rep. 2020 Feb 28;40(2). doi: 10.1042/BSR20193087.
Sperm-associated antigen 5 (SPAG5), a spindle-binding protein, regulates the process of mitosis. The present study focused on the relationship between SPAG5 expression and the clinicopathological characteristics and prognosis of ovarian cancer.
First, we used the Gene Expression Omnibus (GEO) database to analyze SPAG5 expression in ovarian cancer and its clinical relevance. Subsequently, qPCR test was used to detect SPAG5 mRNA expression in 20 cases of ovarian cancer. The expression of SPAG5 protein in a tissue microarray containing 102 cases of ovarian cancer was detected by immunohistochemistry. Cox regression and Kaplan-Meier survival analyses were performed to identify the prognostic factors for the 102 ovarian cancer patients.
In the GEO datasets, SPAG5 mRNA expression was significantly higher in ovarian cancer tissues than that in normal ovarian tissues (P < 0.001). qPCR and immunohistochemistry showed that SPAG5 expression in ovarian cancer tissues was significantly higher than that in paracancerous tissues (P = 0.002, P < 0.001). The high expression of SPAG5 in ovarian cancer was correlated with histological type (P = 0.009), lymph node metastasis (P = 0.001), distant metastasis (P = 0.001), TNM stage (P = 0.001), and prognosis (P = 0.001). The Kaplan-Meier curve indicated that rates of disease-free survival (DFS) and overall survival (OS) were even lower in patients with high SPAG5 expression. Multivariate analysis showed that SPAG5 expression (P = 0.001) and TNM staging (P = 0.002) were independent prognostic factors for the DFS of ovarian cancer.
These results suggest that high SPAG5 expression was correlated with multiple clinicopathological features of ovarian cancer and can be used as an evaluation indicator for a poor ovarian cancer prognosis.
精子相关抗原 5（SPAG5）是一种纺锤体结合蛋白，调节有丝分裂过程。本研究重点关注 SPAG5 表达与卵巢癌临床病理特征和预后的关系。
首先，我们使用基因表达综合数据库（GEO）分析卵巢癌中 SPAG5 的表达及其与临床的相关性。随后，采用 qPCR 检测 20 例卵巢癌中 SPAG5mRNA 的表达。采用免疫组织化学法检测包含 102 例卵巢癌组织微阵列中 SPAG5 蛋白的表达。采用 Cox 回归和 Kaplan-Meier 生存分析确定 102 例卵巢癌患者的预后因素。
在 GEO 数据集，卵巢癌组织中 SPAG5mRNA 的表达明显高于正常卵巢组织（P<0.001）。qPCR 和免疫组化结果显示，卵巢癌组织中 SPAG5 的表达明显高于癌旁组织（P=0.002，P<0.001）。卵巢癌中 SPAG5 的高表达与组织学类型（P=0.009）、淋巴结转移（P=0.001）、远处转移（P=0.001）、TNM 分期（P=0.001）和预后（P=0.001）相关。Kaplan-Meier 曲线表明，SPAG5 高表达患者的无病生存率（DFS）和总生存率（OS）更低。多因素分析显示，SPAG5 表达（P=0.001）和 TNM 分期（P=0.002）是卵巢癌 DFS 的独立预后因素。
这些结果表明，SPAG5 高表达与卵巢癌的多种临床病理特征相关，可作为评估卵巢癌预后不良的指标。
