miR-101、miR-345对乙肝病毒复制调控及肝癌细胞生长的影响。
Effects of miR-101, miR-345 on HBV replication regulation and on the growth of liver cancer cells.
作者信息
Ding Aikun, Wang Cuiyun, Zhang Lihua
机构信息
Department of Infectious Disease, Jinan Infectious Disease Hospital, Jinan, Shandong 250021, P.R. China.
ICU, Jinan Infectious Disease Hospital, Jinan, Shandong 250021, P.R. China.
出版信息
Oncol Lett. 2019 Jan;17(1):1167-1171. doi: 10.3892/ol.2018.9669. Epub 2018 Nov 5.
The aim of the study was to investigate the effects of miRNA-101 and miRNA-345 on HBV replication and liver cancer cell growth. qPCR was performed to detect the expression of miRNA-101 and miRNA-345. The expression of HBV RNA was detected by PCR. The expression of HbsAg was detected using ELISA. BEL-7404 cell line proliferation was detected by MTT assay. The expression levels of miR-101 and miR-345 in BEL-7404 pSUPER.neo-miR-101 group and BEL-7404 pSUPER.neo-miR-345 group were significantly higher than those in BEL-7404 pSUPER.neo group (P<0.05). The expression levels of miR-101 and miR-345 in MHCC97-L pSUPER.neo-miR-101 group and MHCC97-L pSUPER.neo-miR-345 group were significantly higher than those in MHCC97-L pSUPER.neo group (P<0.05). The expression of HBV DNA in MHCC97-L pSUPER.neo-miR-101 group was significantly lower than that in MHCC97-L pSUPER.neo group (P<0.05), and the expression of HBV DNA in MHCC97-L pSUPER.neo-miR-345 group was significantly higher than that in MHCC97-L pSUPER.neo group (P<0.05). The expression of HbsAg in MHCC97-L pSUPER.neo-miR-101 group was significantly lower than that in MHCC97-L pSUPER.neo group (P<0.05), and the expression of HbsAg in MHCC97-L pSUPER.neo-miR-345 group was significantly higher than that in MHCC97-L pSUPER.neo group (P<0.05). There was a significant difference in terms of HbsAg expression between the MHCC97-L pSUPER.neo-miR-101 and MHCC97-L pSUPER.neo-miR-345 groups (P<0.05). The proliferation of BEL-7404 cells in the BEL-7404 pSUPER.neo-miR-101 group was significantly lower than that in the BEL-7404 pSUPER.neo group (P<0.05). The proliferation of BEL-7404 cells in the BEL-7404 pSUPER.neo-miR-345 group was significantly higher than that in the BEL-7404 pSUPER.neo group (P<0.05). The proliferation of BEL-7404 cells in BEL-7404 pSUPER.neo-miR-101 group was different from that in BEL-7404 pSUPER.neo-miR-345 group (P<0.05). miR-101 reduced the level of HBV replication, and inhibited the proliferation of liver cancer cells. miR-345 also upregulated the level of HBV replication, and promoted the proliferation of liver cancer cells.
本研究的目的是探讨miRNA - 101和miRNA - 345对乙肝病毒（HBV）复制及肝癌细胞生长的影响。采用qPCR检测miRNA - 101和miRNA - 345的表达。通过PCR检测HBV RNA的表达。使用ELISA检测乙肝表面抗原（HbsAg）的表达。采用MTT法检测BEL - 7404细胞系的增殖情况。BEL - 7404 pSUPER.neo - miR - 101组和BEL - 7404 pSUPER.neo - miR - 345组中miR - 101和miR - 345的表达水平显著高于BEL - 7404 pSUPER.neo组（P<0.05）。MHCC97 - L pSUPER.neo - miR - 101组和MHCC97 - L pSUPER.neo - miR - 345组中miR - 101和miR - 345的表达水平显著高于MHCC97 - L pSUPER.neo组（P<0.05）。MHCC97 - L pSUPER.neo - miR - 101组中HBV DNA的表达显著低于MHCC97 - L pSUPER.neo组（P<0.05），而MHCC97 - L pSUPER.neo - miR - 345组中HBV DNA的表达显著高于MHCC97 - L pSUPER.neo组（P<0.05）。MHCC97 - L pSUPER.neo - miR - 101组中HbsAg的表达显著低于MHCC97 - L pSUPER.neo组（P<0.05），而MHCC97 - L pSUPER.neo - miR - 345组中HbsAg的表达显著高于MHCC97 - L pSUPER.neo组（P<0.05）。MHCC97 - L pSUPER.neo - miR - 101组和MHCC97 - L pSUPER.neo - miR - 345组在HbsAg表达方面存在显著差异（P<0.05）。BEL - 7404 pSUPER.neo - miR - 101组中BEL - 7404细胞的增殖显著低于BEL - 7404 pSUPER.neo组（P<0.05）。BEL - 7404 pSUPER.neo - miR - 345组中BEL - 7404细胞的增殖显著高于BEL - 7404 pSUPER.neo组（P<0.05）。BEL - 7404 pSUPER.neo - miR - 101组中BEL - 7404细胞的增殖与BEL - 7404 pSUPER.neo - miR - 345组不同（P<0.05）。miR - 101降低了HBV复制水平，并抑制了肝癌细胞的增殖。miR - 345也上调了HBV复制水平，并促进了肝癌细胞的增殖。
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