Serré Jef, Mathyssen Carolien, Ajime Tom Tanjeko, Korf Hannelie, Maes Karen, Heulens Nele, Gysemans Conny, Mathieu Chantal, Vanaudenaerde Bart, Janssens Wim, Gayan-Ramirez Ghislaine
Laboratory of Respiratory Diseases, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium.
Laboratory of Hepatology, Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), KU Leuven, Leuven, Belgium.
Data Brief. 2018 Dec 18;22:703-708. doi: 10.1016/j.dib.2018.12.048. eCollection 2019 Feb.
This article contains data related to the inflammatory cytokine and investigated pathways involved in bacterial clearance reported in "Airway infection with Nontypeable is more rapidly eradicated in vitamin D deficient mice" (Serré et al., 2018) [1]. Vitamin D deficient or sufficient mice were oropharyngeally instilled with 10 NTHi and sacrificed at 4, 8, 24 and 72 h post-infection. We measured proinflammatory cytokines (KC, TNF-α, IL-1β, IL6 and MCP-1) markers of bacterial clearance pathways (myeloid peroxidase, nitric oxide, complement C5a and immunoglobulin A) in bronchoalveolar fluid (BALF) during infection and mRNA expression levels of innate immune defense mechanism markers (mucin glycoproteins, pathogen recognitions receptor TLR2 and TLR4, antimicrobial peptides SLPI, REG3γ, lysozyme, BD-1, BD-2, BD-3 and surfactant proteins SP-A and SP-D) in lung homogenate. Finally, genomic DNA of NTHi (protein D) measured in lung homogenate was used as an indicator of NTHi invasion of alveolar macrophages or epithelial cells.
本文包含与炎症细胞因子相关的数据，以及在《维生素D缺乏小鼠中非典型菌株引起的气道感染清除更快》（塞雷等人，2018年）[1]中报道的参与细菌清除的研究途径。将维生素D缺乏或充足的小鼠经口咽部接种10个非典型流感嗜血杆菌（NTHi），并在感染后4、8、24和72小时处死。我们在感染期间测量了支气管肺泡灌洗液（BALF）中促炎细胞因子（KC、TNF-α、IL-1β、IL-6和MCP-1）、细菌清除途径标志物（髓过氧化物酶、一氧化氮、补体C5a和免疫球蛋白A），以及肺匀浆中固有免疫防御机制标志物（粘蛋白糖蛋白、病原体识别受体TLR2和TLR4、抗菌肽SLPI、REG3γ、溶菌酶、BD-1、BD-2、BD-3和表面活性蛋白SP-A和SP-D）的mRNA表达水平。最后，将肺匀浆中测量的NTHi（蛋白D）基因组DNA用作NTHi侵袭肺泡巨噬细胞或上皮细胞的指标。
