State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, China.
Nanoscale. 2019 Jan 31;11(5):2249-2263. doi: 10.1039/c8nr09096c.
Real-time and accurate measurement of three-dimensional (3D) temperature field gradient maps of cells and tissues would provide an effective experimental method for analyzing the coupled correlation between metabolism and heat, as well as exploring the thermodynamic properties of nanoparticles under complex environments. In this work, a new principle of quantum 3D thermal imaging is proposed. The photoluminescence principle of quantum dots is expounded and CdTe QDs are prepared by aqueous phase synthesis. Fluorescence spectral characteristics of QDs at different temperatures are studied. The optimized algorithm of the optical spot double helix point spread function is proposed to improve the imaging, where optimized light energy increased by 27.36%. The design scheme of a quantum 3D thermal imaging system is presented. The measurement range is (-8 mm, +8 mm). The temperature is calculated according to the temperature-heat curve of quantum dots. The double helix point spread function has converted the defocus distance of QDs into the rotation angle of the double optical spot, thereby determining its position. The experimental results reveal that real-time 3D tracking and temperature measurements of quantum dots at the micro-nanoscale are achieved. Overall, the proposed nano-scale 3D quantum thermal imaging system with high-resolution may provide a new research direction and exploration of many frontier fields.
实时准确地测量细胞和组织的三维（3D）温度场梯度图，将为分析代谢与热的耦合相关性以及探索复杂环境下纳米粒子的热力学性质提供一种有效的实验方法。在这项工作中，提出了量子 3D 热成像的新原理。阐述了量子点的光致发光原理，并通过水相合成法制备了 CdTe QDs。研究了 QDs 在不同温度下的荧光光谱特性。提出了光学光斑双螺旋点扩散函数的优化算法，以提高成像质量，其中优化后的光能增加了 27.36%。提出了量子 3D 热成像系统的设计方案。测量范围为（-8mm，+8mm）。根据量子点的温度-热曲线计算温度。双螺旋点扩散函数将 QDs 的离焦距离转换为双光斑点的旋转角度，从而确定其位置。实验结果表明，实现了量子点在微纳尺度上的实时 3D 跟踪和温度测量。总之，该高分辨率纳米级 3D 量子热成像系统可为许多前沿领域的研究提供新的方向和探索。
