Estupiñán-López Carlos, Dominguez Christian Tolentino, Cabral Filho Paulo E, Fontes Adriana, de Araujo Renato E
Laboratory of Biomedical Optics and Imaging, Federal University of Pernambuco, Recife, PE, Brazil.
Methods Mol Biol. 2014;1199:93-101. doi: 10.1007/978-1-4939-1280-3_7.
An essential parameter to evaluate the light emission properties of fluorophores is the fluorescence quantum yield, which quantify the conversion efficiency of absorbed photons to emitted photons. We detail here an alternative nonfluorescent method to determine the absolute fluorescence quantum yield of quantum dots (QDs). The method is based in the so-called Thermal Lens Spectroscopy (TLS) technique, which consists on the evaluation of refractive index gradient thermally induced in the fluorescent material by the absorption of light. Aqueous dispersion carboxyl-coated cadmium telluride (CdTe) QDs samples were used to demonstrate the Thermal Lens Spectroscopy technical procedure.
评估荧光团发光特性的一个重要参数是荧光量子产率，它量化了吸收光子到发射光子的转换效率。我们在此详细介绍一种用于确定量子点（QD）绝对荧光量子产率的替代非荧光方法。该方法基于所谓的热透镜光谱（TLS）技术，该技术包括评估荧光材料因光吸收而热诱导产生的折射率梯度。使用羧基包覆的碲化镉（CdTe）量子点水相分散样品来演示热透镜光谱技术过程。
