NYU Center for Musculoskeletal Care, 333 East 38th Street, 4th Floor, New York, NY, 10016, USA.
Department of Orthopaedic Surgery, University of Louisville, Louisville, KY, 40202, USA.
Knee Surg Sports Traumatol Arthrosc. 2019 Jun;27(6):1931-1938. doi: 10.1007/s00167-019-05355-z. Epub 2019 Jan 17.
This in vivo histological study using an ovine model evaluated the 90-day healing of unilateral segmental meniscal allograft transplantation.
Fresh-frozen medial menisci were transplanted to replace the right medial meniscus of six female sheep. Tissue healing was evaluated using semi-quantitative, descriptive methods. Formalin-fixed meniscal, distal femur and proximal tibia tissues were evaluated using Rodeo (cellularity/collagen), Ishida (reparative bonding), Collagen I IHC (collagen I), and Mankin (cartilage organization) scores at the medial femoral condyle (MFC) and medial tibial plateau (MTP). Meniscocapsular evaluations were performed at the: (a) peripheral junction; (b) posterior sector-native meniscus junction; (c) anterior sector-native meniscus junction; (d) posterior horn internal control; and (e) anterior horn internal control.
Three animals were euthanized at 39 ± 2.6 days post-surgery because of their knee condition. These animals had moderate Rodeo scores, low Ishida scores, and high Collagen I staining scores indicating moderately high fibrocartilaginous changes, mild or minimal healing and high collagen I content. Cartilage scores were low in the MFC and moderately high in the MTP, indicating mild MFC cartilage changes and moderately high MTP cartilage changes. Full-term (90 day) euthanized animals (n = 3) displayed improving Rodeo scores with mean scores of 3.3 and 3.6 at junctions (B) and (C), respectively. Ishida scores displayed similar improvements at all sectors. Collagen I staining revealed strong (grade 5) levels in all sections, with mean collagen I scores of 5, 5 and 4 for the peripheral (A), posterior (B) and anterior (C) junctions, respectively. Improved healing was observed at each segmental meniscus sector in terminally euthanized animals.
Segmental meniscal allograft transplantation displayed partial healing to remnant meniscal tissue. Further study is needed to better delineate the time needed for complete healing and the joint-loading progression that may enhance it.
本体内组织学研究使用绵羊模型评估了单侧半月板节段同种异体移植 90 天的愈合情况。
将新鲜冷冻的内侧半月板移植以替代 6 只雌性绵羊的右侧内侧半月板。使用半定量描述性方法评估组织愈合情况。使用 Rodeo（细胞/胶原）、Ishida（修复结合）、Collagen I IHC（胶原 I）和 Mankin（软骨组织）评分评估福尔马林固定的半月板、股骨远端和胫骨近端组织，在股骨内侧髁（MFC）和胫骨内侧平台（MTP）处。在以下部位进行半月板-滑膜评估：（a）周围交界处；（b）后扇区-原生半月板交界处；（c）前扇区-原生半月板交界处；（d）后角内对照；和（e）前角内对照。
3 只动物因膝关节状况在术后 39 ± 2.6 天被安乐死。这些动物的 Rodeo 评分中等，Ishida 评分低，Collagen I 染色评分高，表明存在中度纤维软骨变化、轻度或最小的愈合和高胶原 I 含量。MFC 的软骨评分低，MTP 的软骨评分中度高，表明 MFC 软骨轻度变化，MTP 软骨中度高。足月（90 天）安乐死动物（n = 3）显示在交界处（B）和（C）的 Rodeo 评分分别为 3.3 和 3.6，评分逐渐升高。Ishida 评分在所有扇区均有类似改善。Collagen I 染色显示所有切片均呈强（等级 5）水平，在外侧（A）、后（B）和前（C）交界处的胶原 I 评分分别为 5、5 和 4。在终末期安乐死动物的每个半月板节段都观察到改善的愈合。
半月板节段同种异体移植显示对残留半月板组织的部分愈合。需要进一步研究以更好地描绘完全愈合所需的时间以及可能增强愈合的关节负荷进展。
