诱导多能干细胞衍生神经干细胞的单细胞文库制备
Single-Cell Library Preparation of iPSC-Derived Neural Stem Cells.
作者信息
Kim Jeffrey, Daadi Marcel M
机构信息
Southwest National Primate Research Center, Texas Biomedical Research Institute, San Antonio, TX, USA.
Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of Medicine, University of Texas Health Science Center at San Antonio , San Antonio, TX, USA.
出版信息
Methods Mol Biol. 2019;1919:129-143. doi: 10.1007/978-1-4939-9007-8_10.
Single-cell RNA-seq technology allows for the identification of heterogeneous cell populations, measures stochastic gene expressions, and identifies highly variable genes. Thus, with this technology it is possible to identify relevant pathways involved in development or in disease progression. Herein, we describe a protocol to capture and process single-cell transcriptomes that will be used for RNA sequencing. This chapter discusses the use of the Fluidigm C1 System and Integrated Fluidic Circuit microfluidics system, TapeStation 4200, SMART-Seq v4, Nextera XT Library Preparation Kit, and AMPure XP beads.
单细胞RNA测序技术可用于识别异质细胞群体、测量随机基因表达并鉴定高变基因。因此，利用该技术能够识别参与发育或疾病进展的相关通路。在此，我们描述了一种用于捕获和处理将用于RNA测序的单细胞转录组的方案。本章讨论了Fluidigm C1系统和集成流体回路微流控系统、TapeStation 4200、SMART-Seq v4、Nextera XT文库制备试剂盒以及AMPure XP磁珠的使用。
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