Department of Dentistry, Oral Pathology, Federal University of Rio Grande do Norte, Natal, Brazil.
J Periodontal Res. 2019 Aug;54(4):349-355. doi: 10.1111/jre.12635. Epub 2019 Jan 17.
The aim of this study was to evaluate the angiogenesis and lymphangiogenesis in gingival tissue biopsy specimens of individuals with clinically healthy gingiva, chronic gingivitis, and chronic periodontitis (n = 30 per clinical condition).
Histological sections were stained using hematoxylin and eosin as well as immunohistochemically with hematopoietic progenitor cell antigen CD34 and podoplanin (PDPN) antibodies to evaluate the microvascular count, area, and perimeter of blood and lymphatic vessels, respectively.
The results revealed a correlation between the microvascular count of blood and lymphatic vessels (P = 0.03; however, in individuals with chronic periodontitis, fewer lymphatic vessels were present than in the clinically healthy gingival tissue (P = 0.01), which was not observed in the case of microvascular area and perimeter. Podoplanin labeling was present in the epithelium, and the intensity of labeling was positively correlated to the intensity of the inflammatory infiltrate (P = 0.03).
In this study, we concluded that an increase in the number of blood and lymphatic vessels was not observed in bouth gingivitis and periodontitis samples. Podoplanin expression is highly associated with an increased inflammatory infiltration suggesting that PDPN might play an additional role in periodontal disease, other than solely as a lymphangiogenesis marker.
本研究旨在评估患有临床健康牙龈、慢性龈炎和慢性牙周炎的个体的牙龈组织活检标本中的血管生成和淋巴管生成（每个临床状况 n = 30）。
使用苏木精和伊红染色以及造血祖细胞抗原 CD34 和 podoplanin（PDPN）抗体进行组织学切片免疫组织化学染色，以分别评估血管计数、血液和淋巴管的面积和周长。
结果显示，血管计数与血管和淋巴管之间存在相关性（P = 0.03；然而，在患有慢性牙周炎的个体中，淋巴管数量少于临床健康牙龈组织（P = 0.01），而在血管面积和周长方面则没有观察到这种情况。Podoplanin 标记存在于上皮中，并且标记强度与炎症浸润的强度呈正相关（P = 0.03）。
在这项研究中，我们得出结论，在牙龈炎和牙周炎样本中均未观察到血液和淋巴管数量的增加。Podoplanin 的表达与炎症浸润的增加高度相关，这表明 PDPN 可能在牙周病中发挥额外的作用，而不仅仅是作为淋巴管生成标志物。
