Biomedical Science, Faculty of Chemical-Biological Sciences, Autonomous University of Guerrero, 39090, Chilpancingo de los Bravo, Guerrero, Mexico.
Oral and Maxillofacial Pathology, National School of Higher Studies, Leon Unit of the National Autonomous University of Mexico, Leon, Guanajuato, 37684, Mexico.
Diagn Pathol. 2024 Aug 24;19(1):115. doi: 10.1186/s13000-024-01540-y.
Podoplanin (PDPN) is a transmembrane glycoprotein implicated in the pathogenesis of odontogenic lesions (OL). It is localized at the membrane and cytoplasmic level, and its interaction with other proteins could trigger cell proliferation, invasion and migration. The main objective of this systematic review is to explore the immunoexpression pattern of podoplanin in OL. In addition, as secondary objectives, we aimed to compare the immunostaining intensity of PDPN in OL, to analyze its interaction networks by bioinformatic analysis and to highlight its importance as a potential diagnostic marker useful in the pathogenesis of OL.
The protocol was developed following PRISMA and Cochrane guidelines. The digital search was performed in the databases: PubMed/MEDLINE, ScienceDirect, Scopus, Web of Science and Google Schoolar from August 15, 2010 to June 15, 2023. We included cross-sectional and cohort studies that will analyze the pattern of PDPN immunoexpression in OL. Two investigators independently searched for eligible articles, selected titles and abstracts, analyzed full text, conducted data collection, and performed assessment of study quality and risk of bias. In addition, part of the results were summarized through a random-effects meta-analysis. STRING database was used for protein-protein interaction analysis.
Twenty-nine relevant studies were included. The ages of the subjects ranged from 2 to 89 years, with a mean age of 33.41 years. Twenty-two point two percent were female, 21.4% were male, and in 56.4% the gender of the participants was not specified. A total of 1,337 OL samples were analyzed for PDPN immunoexpression pattern. Ninety-four (7.03%) were dental follicles and germs, 715 (53.47%) were odontogenic cysts, and 528 (39.49%) were odontogenic tumors. Meta-analysis indicated that the immunostaining intensity was significantly stronger in odontogenic keratocysts compared to dentigerous cysts (SMD=3.3(CI=1.85-4.82, p=0.000*). Furthermore, bioinformatic analysis revealed that PECAM-1, TNFRF10B, MSN, EZR and RDX interact directly with PDPN and their expression in OL was demonstrated.
The results of the present systematic review support the unique immunoexpression of PDPN as a potential useful diagnostic marker in the pathogenesis of OL.
Podoplanin (PDPN) 是一种参与牙源性病变 (OL) 发病机制的跨膜糖蛋白。它定位于膜和细胞质水平，其与其他蛋白质的相互作用可能触发细胞增殖、侵袭和迁移。本系统评价的主要目的是探讨 PDPN 在 OL 中的免疫表达模式。此外，作为次要目标，我们旨在比较 OL 中 PDPN 的免疫染色强度，通过生物信息学分析分析其相互作用网络，并强调其作为一种潜在的有助于理解 OL 发病机制的有用诊断标志物的重要性。
该方案遵循 PRISMA 和 Cochrane 指南制定。数字检索在数据库中进行：PubMed/MEDLINE、ScienceDirect、Scopus、Web of Science 和 Google Scholar，检索时间为 2010 年 8 月 15 日至 2023 年 6 月 15 日。我们纳入了分析 OL 中 PDPN 免疫表达模式的横断面和队列研究。两名研究人员独立搜索符合条件的文章，选择标题和摘要，分析全文，进行数据收集，并进行研究质量和偏倚风险评估。此外，部分结果通过随机效应荟萃分析进行了总结。STRING 数据库用于蛋白质-蛋白质相互作用分析。
共纳入 29 项相关研究。受试者年龄 2-89 岁，平均年龄 33.41 岁。22.2%为女性，21.4%为男性，56.4%的参与者性别未指定。共有 1337 例 OL 样本用于分析 PDPN 的免疫表达模式。其中 94 例（7.03%）为牙滤泡和牙胚，715 例（53.47%）为牙源性囊肿，528 例（39.49%）为牙源性肿瘤。荟萃分析表明，牙源性角化囊肿的免疫染色强度明显强于含牙囊肿（SMD=3.3（CI=1.85-4.82，p=0.000*）。此外，生物信息学分析显示，PECAM-1、TNFRF10B、MSN、EZR 和 RDX 直接与 PDPN 相互作用，其在 OL 中的表达得到证实。
本系统评价的结果支持 PDPN 作为一种潜在的有用诊断标志物在 OL 发病机制中的独特免疫表达。
