Li Tao, Rong Zhi-Hua, Chang Neng-Bin, Liu Xing, Xu Jia-Ying, Liu Dong, Shi Cong-Cong, Zhang Wen-Yi, Jiang Rui, Jiang Jun
Key Laboratory of Medical Electrophysiology, Ministry of Education, Collaborative Innovation Center for Prevention and Treatment of Cardiovascular Disease/Institution of Cardiovascular Research, Southwest Medical University, Luzhou, China.
Department of Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
J Cell Physiol. 2019 Sep;234(9):15225-15234. doi: 10.1002/jcp.28164. Epub 2019 Jan 17.
Intimal hyperplasia is an important cause of stenosis or occlusion after vascular injury. Circular RNAs (circRNAs) are known to be related to various cardiovascular diseases. However, the expression profile of circRNAs in the neointima has not been reported in detail. In this study, we established a rat common carotid artery (CCA) injury model. A microarray detection showed significant differences in circRNA expression between the normal and injured CCA. Real-time quantitative polymerase chain reaction verified the differences. We used bioinformatics to predict the microRNAs that possibly interact with the differentially expressed (DE) circRNAs and linked the potential functions of circRNAs to the target genes of the microRNAs. We believe that the DE circRNA in neointima may affect the differentiation, proliferation, and migration of vascular cells through a variety of target genes. The intervention or utilization of certain circRNAs should be a new method for preventing and treating intimal hyperplasia.
内膜增生是血管损伤后狭窄或闭塞的重要原因。已知环状RNA（circRNA）与多种心血管疾病有关。然而，circRNA在新生内膜中的表达谱尚未详细报道。在本研究中，我们建立了大鼠颈总动脉（CCA）损伤模型。微阵列检测显示正常和损伤CCA的circRNA表达存在显著差异。实时定量聚合酶链反应验证了这些差异。我们使用生物信息学预测可能与差异表达（DE）circRNA相互作用的微小RNA，并将circRNA的潜在功能与微小RNA的靶基因联系起来。我们认为，新生内膜中的DE circRNA可能通过多种靶基因影响血管细胞的分化、增殖和迁移。对某些circRNA的干预或利用应该是预防和治疗内膜增生的一种新方法。
