Department of Otorhinolaryngology, Wuxi No. 2 People's Hospital, Wuxi, P.R. China.
Eur Rev Med Pharmacol Sci. 2019 Jan;23(1):217-224. doi: 10.26355/eurrev_201901_16767.
This study aims to investigate the effect of miR-26a on the epithelial-mesenchymal transition (EMT) and invasion of Tu686 cell line through SMAD1.
Tu686 Squamous cell carcinoma of head and neck (SCCHN) cell strains were divided into the miR-26a group, miR-NC group, co-transfection group and blank control group. Among them, the miR-26a group only transfected miR-26a mimics, the miR-NC group only transfected miR-26a negative control, the co-transfection group transfected miR-26a mimics and pcDNA3.1-SMAD1 plasmid. The qRT-PCR method was used for the detection of the expressions of miR-26a and SMAD1 in each group of cells, transwell assay for the detection of the invasion ability of each group of cells and Western blot for detecting the expression level of SMAD1 and the expressions of EMT-related proteins E-cadherin and N-cadherin.
The relative expression of miR-26a in the miR-26a group was significantly higher than that in the miR-NC group and blank control group, and the relative expression in the co-transfection group was significantly higher than that in the miR-NC and blank control groups. The relative expression of SMAD1 in the miR-26a group was significantly lower than that in the miR-NC and blank control groups, and the relative expression in the co-transfection group was lower than that in the miR-NC and blank control groups, and higher than that in the miR-26a group (all p<0.05). There was no significant difference between the miR-NC group and the blank control group (p>0.05).
miR-26a may reduce the expression level of SMAD1, affect the expression of EMT-related proteins, inhibit the EMT function of Tu686 cells of squamous cell carcinoma of head and neck, and inhibit the invasion of them.
本研究旨在通过 SMAD1 探讨 miR-26a 对 Tu686 细胞系上皮-间充质转化（EMT）和侵袭的影响。
将头颈部鳞状细胞癌（SCCHN）Tu686 细胞株分为 miR-26a 组、miR-NC 组、共转染组和空白对照组。其中，miR-26a 组仅转染 miR-26a 模拟物，miR-NC 组仅转染 miR-26a 阴性对照物，共转染组转染 miR-26a 模拟物和 pcDNA3.1-SMAD1 质粒。采用 qRT-PCR 法检测各组细胞中 miR-26a 和 SMAD1 的表达，Transwell 法检测各组细胞的侵袭能力，Western blot 法检测 SMAD1 及 EMT 相关蛋白 E-钙黏蛋白和 N-钙黏蛋白的表达水平。
miR-26a 组 miR-26a 的相对表达量明显高于 miR-NC 组和空白对照组，共转染组明显高于 miR-NC 组和空白对照组。miR-26a 组 SMAD1 的相对表达量明显低于 miR-NC 组和空白对照组，共转染组明显低于 miR-NC 组和空白对照组，高于 miR-26a 组（均 P<0.05）。miR-NC 组与空白对照组比较，差异无统计学意义（P>0.05）。
miR-26a 可能通过降低 SMAD1 的表达水平，影响 EMT 相关蛋白的表达，抑制头颈部鳞状细胞癌 Tu686 细胞的 EMT 功能，抑制其侵袭。
