Department of Otolaryngology Head and Neck Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, China.
Cancer Biother Radiopharm. 2019 Apr;34(3):189-195. doi: 10.1089/cbr.2018.2621. Epub 2019 Jan 10.
To investigate the correlations of miR-31 expression with cell proliferation, invasion, and prognosis of patients with head and neck squamous cell carcinoma (HNSCC).
The expression of miR-31 in human laryngeal cancer TU686 cells, human nasopharyngeal carcinoma CNE-2 cells, and normal human oral keratinocyte (NHOK) epithelial cells was detected via quantitative real-time polymerase chain reaction (qRT-PCR). The effects of miR-31 on the proliferation and invasion of HNSCC cells were explored through transfecting miR-31 analogs (miR-31 mimics) and miR-31 inhibitors (anti-miR-31). qRT-PCR was applied to detect the expressions of miR-31 in 56 cases of HNSCC tumor tissues and tumor-adjacent normal tissues. The correlation of miR-31 expression with pathological parameters and survival prognosis of HNSCC patients was also analyzed.
The expressions of miR-31 in TU686 and CNE-2 cell lines were significantly higher than that in NHOK cells (p < 0.01). Compared with those in the negative control group, the proliferation and invasion abilities of cells transfected with miR-31 mimics were notably enhanced (p < 0.01), and those of cells transfected with anti-miR-31 were significantly reduced (p < 0.01). In addition, miR-31 mimics significantly reduced ARID1A expression (p < 0.01) and anti-miR-31 increased its expression (p < 0.05). The expression of miR-31 in tumor tissues of HNSCC patients was remarkably higher than that in tumor-adjacent normal tissues (p < 0.01). This, together with clinical data analysis, revealed that the expression of miR-31 was associated with tumor differentiation, metastasis, and staging of patients, and the survival period of patients with lowly expressed miR-31 was longer.
The highly expressed miR-31 can stimulate the proliferation and invasion of HNSCC cells, closely correlated with tumor differentiation, metastasis, and staging of patients. Patients with lowly expressed miR-31 have a longer survival period. Therefore, miR-31 expression can be taken as a crucial reference indicator for the prognosis of HNSCC patients.
探讨 miR-31 表达与头颈部鳞状细胞癌（HNSCC）患者细胞增殖、侵袭和预后的相关性。
采用实时定量聚合酶链反应（qRT-PCR）检测人喉癌细胞 TU686、人鼻咽癌细胞 CNE-2 和正常人口腔角质细胞（NHOK）上皮细胞中 miR-31 的表达。通过转染 miR-31 模拟物（miR-31 模拟物）和 miR-31 抑制剂（anti-miR-31）探讨 miR-31 对 HNSCC 细胞增殖和侵袭的影响。采用 qRT-PCR 检测 56 例 HNSCC 肿瘤组织和肿瘤旁正常组织中 miR-31 的表达，并分析 miR-31 表达与 HNSCC 患者病理参数和生存预后的相关性。
TU686 和 CNE-2 细胞系中 miR-31 的表达明显高于 NHOK 细胞（p<0.01）。与阴性对照组相比，转染 miR-31 模拟物的细胞增殖和侵袭能力明显增强（p<0.01），而转染 anti-miR-31 的细胞侵袭能力显著降低（p<0.01）。此外，miR-31 模拟物显著降低 ARID1A 表达（p<0.01），anti-miR-31 增加其表达（p<0.05）。HNSCC 患者肿瘤组织中 miR-31 的表达明显高于肿瘤旁正常组织（p<0.01）。结合临床数据分析，结果表明 miR-31 的表达与患者肿瘤分化、转移和分期有关，miR-31 低表达患者的生存期较长。
高表达的 miR-31 可刺激 HNSCC 细胞的增殖和侵袭，与患者肿瘤分化、转移和分期密切相关。miR-31 低表达的患者生存期较长。因此，miR-31 表达可作为 HNSCC 患者预后的重要参考指标。
