Dermatology Hospital of Jiangxi Provinces and Jiangxi Dermatology Institute, Nanchang, China.
Jiangxi Provincial People's Hospital, Nanchang, China.
Med Mycol. 2019 Nov 1;57(8):954-961. doi: 10.1093/mmy/myy163.
Sporotrichosis is a subcutaneous mycosis caused by traumatic inoculation of pathogenic Sporothrix species. Until recently, Sporothrix globosa was considered as the unique Chinese species causing this disorder. In the present study, 33 clinical Sporothrix strains isolated from Jiangxi, China, were classified and antifungal susceptibility for each strain was determined. Thirteen S. globosa strains and 20 S. schenckii strains were identified by morphology and by multilocus analysis using rDNA ITS, CAL, and EF1α (i.e., internal transcribed spacer, calmodulin and elongation factor-1α). In vitro antifungal susceptibility testing of yeast phases indicated that itraconazole, terbinafine, and posaconazole were most effective against both species, followed by amphotericin B and voriconazole, while fluconazole, 5-fluorocytosine had low efficacy with high MICs. Co-occurrence of S. schenckii and S. globosa in central China may indicate different routes of transmission in this area.
申克孢子丝菌病是一种由创伤性接种致病性申克孢子丝菌引起的皮下真菌病。直到最近，球形孢子丝菌才被认为是引起这种疾病的中国特有种。在本研究中，对来自中国江西的 33 株临床申克孢子丝菌进行了分类，并对每种菌株的抗真菌药敏性进行了测定。通过形态学和 rDNA ITS、CAL 和 EF1α（即内部转录间隔区、钙调蛋白和延伸因子-1α）的多位点分析，鉴定出 13 株球形孢子丝菌和 20 株申克孢子丝菌。对酵母相的体外抗真菌药敏试验表明，伊曲康唑、特比萘芬和泊沙康唑对这两种菌最有效，其次是两性霉素 B 和伏立康唑，而氟康唑和 5-氟胞嘧啶疗效较低，MIC 值较高。中国中部申克孢子丝菌和球形孢子丝菌的共存可能表明该地区存在不同的传播途径。
