分析印度健康成年人中的登革热特异性记忆 B 细胞、中和抗体和结合抗体。

Analysis of dengue specific memory B cells, neutralizing antibodies and binding antibodies in healthy adults from India.

机构信息

ICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India; Department of Pediatrics, Division of Infectious Disease, Emory University School of Medicine, Atlanta, GA, USA; Emory Vaccine Center, Emory University School of Medicine, Atlanta, GA, 30322, USA.

ICGEB-Emory Vaccine Center, International Center for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi, 110067, India.

出版信息

Int J Infect Dis. 2019 Jul;84S:S57-S63. doi: 10.1016/j.ijid.2019.01.018. Epub 2019 Jan 15.

DOI:10.1016/j.ijid.2019.01.018

PMID:30658170

Abstract

BACKGROUND

The Indian population is facing highest dengue burden worldwide supporting an urgent need for vaccines. For vaccine introduction, evaluation and interpretation it is important to gain a critical understanding of immune memory induced by natural exposure. However, immune memory to dengue remains poorly characterized in this region.

METHODS

We enumerated levels of dengue specific memory B cells (MBC), neutralizing (NT) and binding antibodies in healthy adults (n=70) from New Delhi.

RESULTS

NT-antibodies, binding antibodies and MBC were detectable in 86%, 86.56% and 81.63% of the subjects respectively. Among the neutralizing positive subjects, 58%, 27%, 5% and 10% neutralized all four, any three, any two and any one dengue serotypes respectively. The presence of the neutralizing antibodies was associated with the presence of the MBC and binding antibodies. However, a massive interindividual variation was observed in the levels of the neutralizing antibodies (range, <1:50-1:30,264), binding antibodies (range, 1:3,000-1:134,000,) as well as the MBC (range=0.006%-5.05%).

CONCLUSION

These results indicate that a vast majority of the adults are immune to multiple dengue serotypes and show massive interindividual variation in neutralizing/binding antibodies and MBCs - emphasizing the importance of monitoring multiple parameters of immune memory in order to properly plan, evaluate and interpret dengue vaccines.

摘要

背景

印度人口是世界上感染登革热负担最高的人群，这迫切需要疫苗的支持。为了疫苗的引入、评估和解释，了解自然感染引起的免疫记忆是非常重要的。然而，该地区对登革热的免疫记忆仍知之甚少。

方法

我们对新德里的 70 名健康成年人进行了登革热特异性记忆 B 细胞（MBC）、中和（NT）和结合抗体水平的检测。

结果

分别有 86%、86.56%和 81.63%的受试者检测到 NT 抗体、结合抗体和 MBC。在中和抗体阳性的受试者中，分别有 58%、27%、5%和 10%的人能中和所有 4 种、任意 3 种、任意 2 种和任意 1 种登革热血清型。中和抗体的存在与 MBC 和结合抗体的存在相关。然而，在中和抗体（范围，<1:50-1:30,264）、结合抗体（范围，1:3,000-1:134,000）以及 MBC（范围=0.006%-5.05%）的水平上，观察到个体间存在巨大的差异。