Fiorati-Aguiar Soraia Monique, Lucisano Marília Pacífico, Silva Léa Assed Bezerra da, Silva Raquel Assed Bezerra da, Spadaro Augusto César Cropanese, Borsatto Maria Cristina, Nelson-Filho Paulo
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Department of Physics and Chemistry, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Am J Dent. 2018 Dec;31(6):279-284.
To evaluate, in vitro, mechanical, chemical and antimicrobial properties of Embrace Wetbond bisphenol A (BPA)-free pit-and-fissure sealant.
For the shear bond strength and microleakage tests, 135 healthy third molars were randomly divided into six groups, according to sealant [Fluroshield (FS) and Embrace Wetbond (EWB)] and contamination conditions (no contamination, contamination with saliva and contamination with water). In addition, EWB sealant was subjected to the following analyses: fluoride release using direct potentiometry, antimicrobial activity using the agar well diffusion method and quantification of BPA using gas chromatography and mass spectrometry. Data were analyzed statistically by ANOVA, Kruskal-Wallis test, Tukey's post-test, Dunn's post-test and Bonferroni post-test (α= 0.05).
For shear bond strength, there was a statistically significant difference among the groups (P< 0.05), and FS presented the best results. Analysis of the microleakage results showed statistically significant differences among the groups (P< 0.05), except for EWB non-contaminated, contaminated with saliva, and contaminated with water (P> 0.05). FS without contamination presented the lowest microleakage of all groups (P< 0.05), followed by FS contaminated with saliva, and three groups in which EWB was used under different conditions. FS contaminated with water presented the highest microleakage (P< 0.05). EWB showed higher fluoride release in water and artificial saliva on the first day (P< 0.05). EWB had greater antimicrobial activity than FS. BPA and Bis-GMA were not detected in EWB. When used according to the manufacturer's instructions (i.e., under conditions of moisture contamination), EWB showed high fluoride release, high antimicrobial activity, no release of BPA or Bis-GMA and shear bond strength close to the minimum acceptable value. However, this sealant presented statistically significantly more microleakage than the other tested materials.
Knowing the adverse effects of BPA in the body, the present study has clinical relevance since it demonstrates some mechanical, chemical and antimicrobial properties of a BPA-free pit-and-fissure sealant.
在体外评估不含双酚A（BPA）的Embrace Wetbond窝沟封闭剂的机械性能、化学性能及抗菌性能。
为进行剪切粘结强度和微渗漏测试，将135颗健康的第三磨牙根据封闭剂类型[氟防护剂（FS）和Embrace Wetbond（EWB）]及污染情况（无污染、唾液污染和水污染）随机分为六组。此外，对EWB封闭剂进行以下分析：采用直接电位法测定氟释放量，采用琼脂扩散法测定抗菌活性，采用气相色谱和质谱法定量分析BPA。数据采用方差分析、Kruskal-Wallis检验、Tukey事后检验、Dunn事后检验和Bonferroni事后检验进行统计学分析（α = 0.05）。
对于剪切粘结强度，各组间存在统计学显著差异（P < 0.05），FS表现出最佳结果。微渗漏结果分析显示，各组间存在统计学显著差异（P < 0.05），但EWB无污染、唾液污染和水污染组除外（P > 0.05）。未受污染的FS在所有组中微渗漏最低（P < 0.05），其次是唾液污染的FS，以及在不同条件下使用EWB的三组。水污染的FS微渗漏最高（P < 0.05）。EWB在第一天在水和人工唾液中的氟释放量更高（P < 0.05）。EWB的抗菌活性高于FS。在EWB中未检测到BPA和双酚A缩水甘油醚（Bis-GMA）。按照制造商说明使用时（即在有水分污染的条件下），EWB显示出高氟释放量、高抗菌活性、不释放BPA或Bis-GMA且剪切粘结强度接近最低可接受值。然而，这种封闭剂的微渗漏在统计学上显著高于其他测试材料。
鉴于了解到BPA对人体的不良影响，本研究具有临床相关性，因为它展示了一种不含BPA的窝沟封闭剂的一些机械性能、化学性能及抗菌性能。
