Velo Marilia Mattar de Amoêdo Campos, Magalhães Ana Carolina, Shiota Aymee, Farha Ana Laura Herrera, Grizzo Larissa Tercilia, Honório Heitor Marques, Wang Linda
Department of Operative Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, São Paulo, Brazil.
Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, São Paulo, Brazil.
Am J Dent. 2018 Dec;31(6):290-296.
This in vitro study was conducted in two regimens hypothesizing that high-fluoride (F) toothpaste (5,000 µg F/g) with or without functionalized tri-calcium phosphate (f TCP) would provide additional protection against (1) root caries development and (2) progression. For both regimens, bovine root dentin specimens were used.
In regimen 1, 60 specimens were subjected daily to alternate immersions between demineralizing and remineralizing solutions for 7 days. The specimens were randomly distributed into four treatment groups (n=15) : non-F toothpaste; Colgate Total 12 (CT) (1,450 µg F/g); Colgate Prevident (CP) (5,000 µg F/g); and Clinpro 5000 (CL) (5,000 µg F/g) associated with (f TCP), and treated with toothpastes/water slurries (1:3 w/w) (5 minutes), twice a day. In regimen 2, the efficacy of the toothpastes to inhibit the progression of the developed lesions was assessed, using the previously described pH-cycling model without additional treatment. The response variables were based on the percentage of surface hardness change (ΔSH), cross-sectional hardness (ΔCSH), mineral content/lesion depth by transversal microradiography (TMR) and the alkali-soluble F uptake.
Both high-F toothpastes reduced initial dentin demineralization and increased the F uptake compared to non-F and conventional toothpastes (P< 0.05). In terms of caries lesion progression, all tested toothpastes were able to promote this protection.
High-F toothpastes, regardless of the presence of f TCP, were more effective than conventional F toothpaste in inhibiting initial root dentin demineralization.
本体外研究采用两种方案进行，假设含或不含功能化磷酸三钙（f TCP）的高氟（F）牙膏（5000μg F/g）能为预防（1）牙根龋发展和（2）进展提供额外保护。两种方案均使用牛牙根牙本质标本。
在方案1中，60个标本每天在脱矿溶液和再矿化溶液之间交替浸泡7天。标本被随机分为四个处理组（n = 15）：不含氟牙膏；高露洁全效12（CT）（1450μg F/g）；高露洁防敏牙膏（CP）（5000μg F/g）；以及与（f TCP）相关的Clinpro 5000（CL）（5000μg F/g），每天两次用牙膏/水浆（1:3 w/w）处理（5分钟）。在方案2中，使用先前描述的pH循环模型，在不进行额外处理的情况下评估牙膏抑制已形成病变进展的效果。反应变量基于表面硬度变化百分比（ΔSH）、横截面硬度（ΔCSH）、横向显微放射照相法（TMR）测量的矿物质含量/病变深度以及碱溶性氟摄取量。
与不含氟和传统牙膏相比，两种高氟牙膏均降低了初始牙本质脱矿并增加了氟摄取（P < 0.05）。在龋损进展方面，所有测试牙膏均能促进这种保护作用。
无论是否存在f TCP，高氟牙膏在抑制初始牙根牙本质脱矿方面均比传统含氟牙膏更有效。
