Laboratory of Veterinary Pharmacokinetics and Pharmacodynamics (LVPP), College of Veterinary Medicine, Kyungpook National University, 41566, 80 Daehakro, Bukgu, Daegu, Republic of Korea.
Forest Resources Development Institute of Gyeongsangbuk-do, Andong, Gyeongsangbuk-do, 36605, Republic of Korea.
BMC Complement Altern Med. 2019 Jan 18;19(1):25. doi: 10.1186/s12906-018-2426-z.
A combination of parts of Cornus officinalis, Rosa multiflora, Lespedeza bicolor, Platycladus orientalis, and Castanea crenata is commonly used for alleviating inflammatory skin disorders. Therefore, this study was carried out to evaluate the in vitro and in vivo preventive effects of a novel herbal formula made from the five plants (C2RLP) against atopic dermatitis in BALB/C mice.
Mice were allocated into five groups (n = 8) including, control (Normal, petrolatum, and betamethasone treated) and treatment groups (treated with 2.5 and 5% C2RLP ointment). Atopic lesion was induced by applying 1-Chloro-2, 4-dinitrobenzene to the dorsal thoracic area of mice. Macroscopical and histological evaluations were performed to determine the effects of treatment on the progress of the skin lesions. The effects of treatment on the production and release of interleukins, interferon -ϒ, nitrite, prostaglandin E2, thymus and activation-receptor chemokine, and β-hexosaminidase were evaluated and comparisons were made between groups. In addition, the chemical compounds present in C2RLP were identified by Liquid Chromatography-Mass Spectrometry.
Topical application of C2RLP reduced the dermatitis score and suppressed histopathological changes in mice. Treatment significantly reduced (P < 0.05) plasma IL-4 level, the production of nitrite, prostaglandin E2, and thymus and activation-receptor chemokine production. The lipopolysaccharide-induced iNOS-mRNA expression in RAW 264.7 cells was also suppressed by high concentrations of C2RLP. In addition, C2RLP showed an inhibitory effect against DPPH free radical (IC = 147.5 μg/ml) and β-hexosaminidase release (IC = 179.5 μg/ml). Liquid Chromatography-Mass Spectrometry analysis revealed the presence of various compounds, including loganin, ellagic acid, and kaempferol 3-glucoside.
Down-regulation of T- helper 2 cellular responses and suppression of inflammatory mediators contributed to the protective effects of C2RLP from atopic dermatitis in BALB/C mice.
由山茱萸、玫瑰、胡枝子、侧柏和栗实组成的复方常用于缓解炎症性皮肤疾病。因此，本研究旨在评估一种由这 5 种植物组成的新型草药配方（C2RLP）对 BALB/C 小鼠特应性皮炎的体外和体内预防作用。
将小鼠分为 5 组（每组 8 只），包括对照组（正常、凡士林和倍他米松处理）和治疗组（用 2.5%和 5% C2RLP 软膏处理）。用 1-氯-2,4-二硝基苯处理小鼠背部胸区以诱导特应性皮炎病变。进行宏观和组织学评估以确定治疗对皮肤病变进展的影响。评估治疗对白细胞介素、干扰素-γ、亚硝酸盐、前列腺素 E2、胸腺激活受体趋化因子和β-己糖胺酶的产生和释放的影响，并比较各组之间的差异。此外，通过液相色谱-质谱法鉴定 C2RLP 中的化学化合物。
C2RLP 的局部应用降低了小鼠的皮炎评分并抑制了其组织病理学变化。治疗组显著降低（P<0.05）了血浆 IL-4 水平、亚硝酸盐、前列腺素 E2 和胸腺激活受体趋化因子的产生。高浓度的 C2RLP 还抑制了 RAW 264.7 细胞中脂多糖诱导的 iNOS-mRNA 表达。此外，C2RLP 对 DPPH 自由基（IC=147.5μg/ml）和β-己糖胺酶释放（IC=179.5μg/ml）具有抑制作用。液相色谱-质谱分析显示存在各种化合物，包括马钱苷、鞣花酸和山奈酚 3-葡萄糖苷。
下调 T 辅助 2 细胞反应和抑制炎症介质有助于 C2RLP 对 BALB/C 小鼠特应性皮炎的保护作用。
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