Pokharel Yuba Raj, Lim Sung Chul, Kim Sang Chan, Choi Hoo Kyun, Kang Keon Wook
13College of Pharmacy, Chosun University, Gwangju, 501-759 Korea.
23Department of Pathology, College of Medicine, College of Medicine, Chosun University, Gwangju, 501-759 Korea.
Toxicol Res. 2008 Mar;24(1):17-22. doi: 10.5487/TR.2008.24.1.017. Epub 2008 Mar 1.
Sopungsan (SS) is a traditional Korean decoction used for the treatment of dermatitis. The aim of this study is to confirm whether or not SS has a preventive effect on the development of atopic dermatitis in dinitrochlorobenzene-applied Nc/Nga mice. SS was administered orally to Nc/Nga mice, which led to the remarkable suppression of the development of dermatitis, as determined by a histological examination and the serum IgE levels. Moreover, SS inhibited the production of thymus- and activation-regulated chemokine (TARC) and its mRNA expression in a keratinocyte cell line, HaCaT, which had been stimulated with tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). Activation of the nuclear factor-κB (NF-κB) or activator protein-1 (AP-1) is one of key steps in the signaling pathways mediating induction of TARC. In this study, SS selectively suppressed NF-κB activation which may be essential for TARC expression in TNF-α/IFN-γ treated keratinocytes. The inhibitory effect of SS on NF-κB activation and TARC production might be associated with the anti-dermatitic effects of SS.
雪峰山（SS）是一种用于治疗皮炎的传统韩国煎剂。本研究的目的是确认SS对二硝基氯苯诱导的Nc/Nga小鼠特应性皮炎的发生是否具有预防作用。将SS口服给予Nc/Nga小鼠，通过组织学检查和血清IgE水平测定，发现其可显著抑制皮炎的发生。此外，SS抑制了角质形成细胞系HaCaT中胸腺和活化调节趋化因子（TARC）的产生及其mRNA表达，该细胞系已用肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）刺激。核因子-κB（NF-κB）或活化蛋白-1（AP-1）的激活是介导TARC诱导的信号通路中的关键步骤之一。在本研究中，SS选择性地抑制了NF-κB的激活，这可能是TNF-α/IFN-γ处理的角质形成细胞中TARC表达所必需的。SS对NF-κB激活和TARC产生的抑制作用可能与其抗皮炎作用有关。
