College of Food Science and Technology, Henan University of Technology, Lianhua Street, Zhengzhou 450001, PR China.
College of Food Science and Technology, Henan University of Technology, Lianhua Street, Zhengzhou 450001, PR China.
Food Chem. 2019 May 30;281:140-146. doi: 10.1016/j.foodchem.2018.12.088. Epub 2018 Dec 30.
For simultaneous determination of lignans (i.e. sesamin, sesamolin and sesamol) in sesame oils, a highly efficient ultrasonic-assisted liquid-liquid microextraction (UALLME) technique was developed based on deep eutectic solvents (DESs) composed of choline chloride and p-cresol. Compared with the common polyols-based DESs (i.e. choline chloride-ethylene glycol), the phenolic DESs exhibited higher extraction efficiency for both polar lignans (i.e. sesamol) and non-polar lignans (i.e. sesamin and sesamolin) due to the π-π interaction between lignans and DES. After the UALLME process, DES phase was analyzed by HPLC-UV. Under optimized condition, all three lignans showed good linear calibration curves and low limit of detection. The recoveries were in the range of 97.3-120%, and the intra-day and inter-day precisions were less than 0.97%. Moreover, this method was used for simultaneous determination of lignans in 15 commercial oil samples. The results showed that the new microextraction technique achieved similar efficiency as the conventional liquid-liquid extraction method.
为了同时测定芝麻油中的木脂素（即芝麻素、芝麻林素和芝麻酚），本研究基于由氯化胆碱和对甲酚组成的深共晶溶剂（DESs）开发了一种高效的超声辅助液-液微萃取（UALLME）技术。与常见的多元醇基 DESs（即氯化胆碱-乙二醇）相比，由于木脂素和 DES 之间的π-π相互作用，酚类 DESs 对极性木脂素（即芝麻酚）和非极性木脂素（即芝麻素和芝麻林素）均表现出更高的萃取效率。在 UALLME 过程之后，通过 HPLC-UV 分析 DES 相。在优化条件下，所有三种木脂素均表现出良好的线性校准曲线和低检测限。回收率在 97.3%-120%范围内，日内和日间精密度均小于 0.97%。此外，该方法还用于同时测定 15 个商业油样中的木脂素。结果表明，新的微萃取技术与传统的液-液萃取方法具有相似的效率。
