Liu Wei, Zong Bingyue, Wang Xiaoping, Cai Junlan, Yu Jingjing
Grain & Corn Engineering Technology Research Center (State Administration of Grain), College of Food Science and Technology, Henan University of Technology Lianhua Street Zhengzhou 450001 P. R. China
Quality and Technique Supervision, Inspection and Testing Center of Xuchang City Xuchang 461000 P. R. China.
RSC Adv. 2019 Jun 4;9(30):17432-17439. doi: 10.1039/c9ra01405e. eCollection 2019 May 29.
A natural deep eutectic solvent (NADES) composed of choline chloride (ChCl) and sesamol was successfully employed in the vortex-assisted liquid-liquid microextraction (VALLME) of food toxicant Sudan I (1-phenylazo-2-naphthalenol) in food samples for HPLC-UV analysis. Sesamol-based NADESs exhibited better Sudan I extraction abilities than other deep eutectic solvents and conventional organic solvents. H NMR and 2D NOESY spectra were used to characterize the sesamol-based NADESs, indicating that hydrogen bonds were formed between ChCl and sesamol. The developed VALLME method showed a high extraction efficiency (near 100%) within 60 s at room temperature. Under the optimized extraction conditions, this established method showed good linearity ( = 1.000) and a low limit of detection (LOD) of 0.02 mg kg. The recoveries were in the range of 93-118%, and the intra-day and inter-day precisions were less than 4.5%. The developed method was successfully applied to the determination of Sudan I in various food samples, including chili oil, chili sauce, and duck egg yolk. This method gave a higher recovery than that of the EU recommended method when applied to sample analysis.
一种由氯化胆碱（ChCl）和芝麻酚组成的天然深共熔溶剂（NADES）成功用于食品样品中食品毒物苏丹红I（1-苯基偶氮-2-萘酚）的涡旋辅助液液微萃取（VALLME），用于高效液相色谱-紫外分析。基于芝麻酚的NADESs对苏丹红I的萃取能力优于其他深共熔溶剂和传统有机溶剂。利用核磁共振氢谱（¹H NMR）和二维核欧沃豪斯效应光谱（2D NOESY）对基于芝麻酚的NADESs进行表征，表明ChCl与芝麻酚之间形成了氢键。所建立的VALLME方法在室温下60 s内显示出高萃取效率（接近100%）。在优化的萃取条件下，该方法具有良好的线性（r² = 1.000）和0.02 mg/kg的低检测限（LOD）。回收率在93% - 118%范围内，日内和日间精密度均小于4.5%。所建立的方法成功应用于辣椒油、辣椒酱和鸭蛋黄等各种食品样品中苏丹红I的测定。该方法应用于样品分析时，回收率高于欧盟推荐方法。
