Hadjiishy P, Renais J, Scebat L
Paroi Arterielle. 1978 Jan-Feb;4(2):111-7.
Thirty0seven rats were given Vitamin D2 orally at the daily dose of 4000 I.U. for two months, then 8000 I.U. for the following two months and 26 rats were used as controls. Batches of both intoxicated and control rats were sacrificed on the 15, 30, 45, 90 and 120 days after the onset of the experiment. This chronic calciferol intoxication allowed a better understanding of the calcifying process. Modified myocytes were observed early and for a long time in and around the mineralizing foci. They had the ultrastructural and histochemical characteristics of cells with an activated metabolism: excessive development of the organelles linked with synthesis processes, hyperactivity of phoshatases, of lysosomic enzymes and of enzymatic systems of cell respiration and glycolysis. Some data of the present study support the hypothesis that myocytes may be a local factor for the aortic calcification process: by increasing the conjonctive constituents calciphily, by producing phosphate and sulphate anions as well as calcifiable organic materials (glycoproteins, GAG), by modification in their own environmental physico-chemical conditions favorous to calcium precipitation (acidosis, phosphate and sulphate anions supersaturation).
307只大鼠口服维生素D2，每日剂量为4000国际单位，持续两个月，随后两个月剂量为8000国际单位，26只大鼠作为对照。在实验开始后的第15、30、45、90和120天，对中毒组和对照组大鼠分批进行处死。这种慢性维生素D中毒有助于更好地理解钙化过程。在矿化灶内及周围早期且长期观察到了形态改变的心肌细胞。它们具有代谢活化细胞的超微结构和组织化学特征：与合成过程相关的细胞器过度发育，磷酸酶、溶酶体酶以及细胞呼吸和糖酵解酶系统的活性增强。本研究的一些数据支持以下假设：心肌细胞可能是主动脉钙化过程的局部因素，通过增加结缔组织成分的嗜钙性，通过产生磷酸根和硫酸根阴离子以及可钙化的有机物质（糖蛋白、糖胺聚糖），通过改变其自身有利于钙沉淀的环境物理化学条件（酸中毒、磷酸根和硫酸根阴离子过饱和）。
