Clinique Ovo, Ovo Labo, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada.
Clinique Ovo, Ovo R and D, 8000 Décarie Boulevard, Suite 600, Montréal Quebec H4P 2S4, Canada.
Reprod Biomed Online. 2019 Mar;38(3):321-329. doi: 10.1016/j.rbmo.2018.12.039. Epub 2019 Jan 5.
Does autologous endometrial cell co-culture (AECC) improve the number of good-quality blastocysts obtained by IVF/intracytoplasmic sperm injection (ICSI), compared with conventional embryo culture medium in a broad group of patients referred to assisted reproductive technology (ART)?
This interventional, randomized, double-blind study took place at Clinique Ovo from March 2013 to October 2015 and included 207 healthy patients undergoing an IVF or ICSI protocol, of which 71 were excluded before randomization. On the previous cycle, all participants underwent an endometrial biopsy at D5 to D7 post-ovulation, following which the endometrial cells were prepared for AECC.
The data demonstrated that AECC significantly increased the incidence of good-quality blastocysts compared with culture in conventional media (42.6% vs 28.4%, P < 0.001). No significant differences were found in pregnancy and live birth rates.
This study demonstrated the benefits of AECC on blastocyst quality compared with conventional embryo culture medium, in a broader category of patients referred to ART as opposed to other studies that concentrated on specific causes of infertility only. However, limitations of the study design should be taken into consideration; the analysis was performed using embryos rather than patients and a follow-up of children born following the treatments could not be conducted.
与传统胚胎培养液相比，自体子宫内膜细胞共培养（AECC）是否能增加接受辅助生殖技术（ART）治疗的广泛患者群体中通过体外受精/胞浆内单精子注射（ICSI）获得的优质囊胚数量？
这项干预性、随机、双盲研究于 2013 年 3 月至 2015 年 10 月在 Clinique Ovo 进行，共纳入 207 例健康接受 IVF 或 ICSI 方案的患者，其中 71 例在随机分组前被排除。在上一个周期中，所有参与者在排卵后第 5 天至第 7 天进行子宫内膜活检，随后准备 AECC 的子宫内膜细胞。
数据表明，AECC 与传统培养基相比，显著增加了优质囊胚的发生率（42.6%比 28.4%，P<0.001）。妊娠率和活产率无显著差异。
与仅针对特定不孕原因的其他研究相比，这项研究表明 AECC 对囊胚质量的有益影响，在接受 ART 治疗的更广泛的患者群体中，而不是其他研究。然而，应考虑研究设计的局限性；该分析是使用胚胎而不是患者进行的，并且无法对接受治疗后出生的儿童进行随访。
