School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok 10150, Thailand.
Ecotoxicol Environ Saf. 2019 Apr 30;171:805-812. doi: 10.1016/j.ecoenv.2019.01.028. Epub 2019 Jan 17.
Eighteen plant species were screened for ozone (O) removal in a continuous system. Zamioculcas zamiifolia had the highest O removal efficiency. To enhance O removal by Z. zamiifolia, adding a compatible endophytic bacteria, Bacillus cereus ERBP into Z. zamiifolia was studied. After operating under an O continuous system (150-250 ppb) at a flow rate of 0.3 L min for 80 h, inoculated plants (74%) exhibited higher O removal efficiency than non-inoculated ones (53%). In addition, after O exposure (80 h), the population of B. cereus ERBP in inoculated plants was significantly increased in both shoots approximately 35 folds and leaves 13 folds compared to inoculated plants without O exposure. The results also showed that B. cereus ERBP had the ability to protect Z. zamiifolia against O stress conditions. The increase in B. cereus ERBP populations was attributed to the significant increase in ascorbate peroxidase (APX) and catalase (CAT) activity. In addition, increasing B. cereus ERBP populations led to raise total flavonoid contents which is one of antioxidant compounds. Increasing APX, CAT activities, and total flavonoid contents can enhance O detoxification in plant tissues. The mechanism of B. cereus ERBP for enhancing O phytoremediation was proposed in this study. The results suggested that B. cereus ERBP was a potential tool for alleviating O stress on Z. zamiifolia and enhancing O phytoremediation efficiency.
十八种植物在连续系统中被筛选用于去除臭氧 (O)。Zamioculcas zamiifolia 的 O 去除效率最高。为了提高 Z. zamiifolia 的 O 去除效率，研究了向其添加相容的内生细菌 Bacillus cereus ERBP。在连续 O 系统（150-250 ppb）下以 0.3 L/min 的流速运行 80 h 后，接种植物（74%）比未接种植物（53%）表现出更高的 O 去除效率。此外，在 O 暴露（80 h）后，接种植物的 Bacillus cereus ERBP 种群在茎中增加了约 35 倍，在叶中增加了 13 倍，而未暴露于 O 的接种植物中则增加了约 35 倍。结果还表明，Bacillus cereus ERBP 具有保护 Z. zamiifolia 免受 O 胁迫的能力。Bacillus cereus ERBP 种群的增加归因于抗坏血酸过氧化物酶 (APX) 和过氧化氢酶 (CAT) 活性的显著增加。此外，Bacillus cereus ERBP 种群的增加导致总类黄酮含量增加，这是一种抗氧化化合物。增加 APX、CAT 活性和总类黄酮含量可以增强植物组织中的 O 解毒。本研究提出了 Bacillus cereus ERBP 增强 O 植物修复的机制。结果表明，Bacillus cereus ERBP 是缓解 Z. zamiifolia O 胁迫和提高 O 植物修复效率的潜在工具。
