School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China; The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, PR China.
Ecotoxicol Environ Saf. 2019 Apr 30;171:833-842. doi: 10.1016/j.ecoenv.2019.01.040. Epub 2019 Jan 17.
In this study, the characteristics of biodegradation of oxytetracycline (OTC) by strain Ochrobactrum sp. KSS10 were studied under various environmental conditions, including initial OTC concentrations, variable temperature, initial pH, and diverse carbon sources. The capability of this bacterial strain for performing simultaneous OTC degradation and nitrate reduction was also explored under aerobic conditions. An OTC degradation ratio of 63.33% and a nitrate removal ratio of 98.64% were obtained within 96 h. In addition, removal of OTC and ammonia from synthetic aquaculture wastewater by a Moving Bed Biofilm Reactor (MBBR) and changes in the resistant genes of microbial communities were also investigated. The results demonstrated that the strain KSS10 was the dominant contributor in OTC and ammonia removal in the MBBR chamber. It removed almost all ammonia and approximately 76.42% of OTC. The abundances of genes tetL, tetX and intI1 were reduced by the MBBR, but the abundance of tetG and tetM were increased due to horizontal and vertical gene transfers. Such a result can potentially be used by the strain KSS10 for removing antibiotics and nitrogen from aquaculture wastewater during pre-treatment.
在这项研究中，研究了在不同环境条件下，包括初始 OTC 浓度、变温、初始 pH 值和不同碳源下，欧文氏菌 KSS10 菌株对土霉素（OTC）生物降解的特性。还探索了该细菌菌株在有氧条件下同时进行 OTC 降解和硝酸盐还原的能力。在 96 小时内，获得了 63.33%的 OTC 降解率和 98.64%的硝酸盐去除率。此外，还研究了移动床生物膜反应器（MBBR）中从合成养殖废水中去除 OTC 和氨以及微生物群落抗性基因的变化。结果表明，KSS10 菌株是 MBBR 腔室中 OTC 和氨去除的主要贡献者。它几乎去除了所有的氨和大约 76.42%的 OTC。MBBR 降低了 tetL、tetX 和 intI1 基因的丰度，但由于水平和垂直基因转移，tetG 和 tetM 基因的丰度增加。这种结果可以使 KSS10 菌株在预处理过程中从养殖废水中去除抗生素和氮。
