School of Life Science, Huizhou University, Huizhou 516007, China.
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Biomed Res Int. 2020 Feb 17;2020:1537873. doi: 10.1155/2020/1537873. eCollection 2020.
In this study, a yeast strain with an outstanding NH-N degradation ability was isolated from the sediment of a black-odor water channel in Guangdong Province, China. Based on phenotypic and phylogenetic analysis, this strain was identified as GW1. The optimum conditions for NH-N degradation by the GW1 strain were as follows: 0.3% inoculum concentration, 1.5 L/min aeration, pH 7, and a temperature of 35°C. Under optimized conditions, the GW1 strain degraded 95.5% of the NH-N. The strain was then added to simulated black-odor water under optimal degradation conditions to investigate changes to the bacterial community over time. 16S rRNA sequencing of samples collected on days 0, 7, 14, and 21 showed that, in the presence of the GW1 strain, the relative abundances of the phyla Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes increased in the black-odor water. In addition, the relative abundance of , a known NH-N degrading genus, increased. This study will facilitate the use of microbiological methods to repair black-odor water.
本研究从广东省黑臭河道底泥中分离得到一株具有高效 NH-N 降解能力的酵母菌株，命名为 GW1。通过表型和系统发育分析，该菌株被鉴定为酿酒酵母。GW1 菌株降解 NH-N 的最适条件为：接种量 0.3%，通气量 1.5 L/min，pH 值 7，温度 35°C。在最适条件下，GW1 菌株可将 95.5%的 NH-N 降解。然后将该菌株添加到模拟黑臭水中，在最佳降解条件下研究细菌群落随时间的变化。对 0、7、14 和 21 天采集的样品进行 16S rRNA 测序，结果表明，在 GW1 菌株存在的情况下，黑臭水中的优势菌门 Proteobacteria、Bacteroidetes、Chloroflexi 和 Firmicutes 的相对丰度增加，同时，已知的 NH-N 降解菌属的相对丰度也增加。本研究将有助于利用微生物方法修复黑臭水。
