脂肪组织氧化应激产物谱因解剖部位而异，并可被大鼠饮食中的亚油酸所改变。
Adipose tissue oxylipin profiles vary by anatomical site and are altered by dietary linoleic acid in rats.
机构信息
Department of Food and Human Nutritional Sciences, University of Manitoba, Canada; Canadian Centre for Agri-Food Research in Health and Medicine, Winnipeg, Canada.
Department of Food and Human Nutritional Sciences, University of Manitoba, Canada; School of Medicine, Federal University of Uberlândia, Brazil.
出版信息
Prostaglandins Leukot Essent Fatty Acids. 2019 Feb;141:24-32. doi: 10.1016/j.plefa.2018.12.004. Epub 2018 Dec 27.
Dietary PUFA and their effects on adipose tissue have been well studied, but oxylipins, the oxygenated metabolites of PUFA, have been sparsely studied in adipose tissue. To determine the oxylipin profile and to examine their potential importance in various adipose sites, female and male rats were provided control, high linoleic acid (LA), or high LA and high α-linolenic acid (LA + ALA) diets for six weeks. Analysis of gonadal (GAT), mesenteric (MAT), perirenal (PAT), and subcutaneous adipose tissues (SAT) revealed higher numbers of oxylipins in MAT and SAT, primarily due to 20-22 carbon cytochrome P450 oxylipins, as well as metabolites of cyclooxygenase derived oxylipins. LA oxylipins made up 75-96% of the total oxylipin mass and largely determined the total relative amounts between depots (GAT > MAT > PAT > SAT). However, when the two most abundant LA oxylipins (TriHOMEs) were excluded, MAT had the highest mass of oxylipins and exhibited the most sex differences. These differences existed despite comparable PUFA composition between depots. Dietary LA increased oxylipins derived from n-6 PUFA, and the addition of ALA generally returned n-6 PUFA oxylipins to levels similar to control and elevated some n-3 oxylipins. These data on oxylipin profiles in adipose depots from different anatomical sites and the effects of diet and sex provide fundamental knowledge that will aid future studies investigating the physiological effects of adipose tissue.
人们对多不饱和脂肪酸（PUFA）及其对脂肪组织的影响进行了广泛研究，但对脂肪组织中多不饱和脂肪酸的氧化代谢产物——氧合脂质（oxylipins）的研究却很少。为了确定氧合脂质的特征，并研究其在不同脂肪组织部位的潜在重要性，雌性和雄性大鼠分别给予对照、高亚油酸（LA）、高 LA 和高 α-亚麻酸（LA+ALA）饮食 6 周。对性腺（GAT）、肠系膜（MAT）、肾周（PAT）和皮下脂肪组织（SAT）进行分析，发现 MAT 和 SAT 中的氧合脂质数量更多，主要是由于 20-22 碳细胞色素 P450 氧合脂质，以及环氧化酶衍生氧合脂质的代谢产物。LA 氧合脂质占总氧合脂质质量的 75-96%，在很大程度上决定了不同脂肪组织之间的总相对含量（GAT＞MAT＞PAT＞SAT）。然而，当排除两种最丰富的 LA 氧合脂质（TriHOMEs）时，MAT 的氧合脂质质量最高，且表现出最大的性别差异。尽管不同部位的多不饱和脂肪酸组成相似，但仍存在这些差异。LA 饮食增加了源自 n-6 多不饱和脂肪酸的氧合脂质，而添加 ALA 通常会使 n-6 多不饱和脂肪酸氧合脂质的水平恢复到对照和升高一些 n-3 氧合脂质的水平。这些关于不同解剖部位脂肪组织中氧合脂质谱以及饮食和性别影响的数据，为研究脂肪组织的生理效应提供了基础知识。
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