Timoshicheva Tatyana A, Zabrodskaya Yana A, Ramsay Edward, Amosova Irina V
Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia.
Smorodintsev Research Institute of Influenza, Russian Ministry of Health, 197376, prof. Popov St. 15/17, St. Petersburg, Russia; Petersburg Nuclear Physics Institute named after B. P. Konstantinov of National Research Center "Kurchatov Institute", 188300, mkr. Orlova roshcha 1, Gatchina, Russia.
Biologicals. 2019 Mar;58:44-49. doi: 10.1016/j.biologicals.2019.01.006. Epub 2019 Jan 17.
In this work, monoclonal antibodies to the adenovirus protein hexon were produced, and their biological and diagnostic properties were characterized. The specific activities of the new monoclonal antibodies, with respect to various adenovirus types, were studied by enzyme-linked immunosorbent assay, indirect immunofluorescence, and western blot analysis. The data demonstrate the potential of the monoclonal antibodies developed, namely 4B7 and 6B12, for use in the development of modern diagnostic assays.
在这项工作中，制备了针对腺病毒六邻体蛋白的单克隆抗体，并对其生物学和诊断特性进行了表征。通过酶联免疫吸附测定、间接免疫荧光和蛋白质印迹分析研究了新型单克隆抗体针对各种腺病毒类型的特异性活性。数据表明所开发的单克隆抗体，即4B7和6B12，在现代诊断检测方法开发中的应用潜力。
