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miR-33b 的上调通过抑制 Wnt/β-连环蛋白信号通路和 ZEB1 表达促进子宫内膜异位症。

Upregulation of miR‑33b promotes endometriosis via inhibition of Wnt/β‑catenin signaling and ZEB1 expression.

机构信息

Department of Gynecology, Affiliated Qilu Hospital of Shandong University, Jinan, Shandong 250002, P.R. China.

Department of Gynecology Ward 1, Linyi City People's Hospital, Linyi, Shandong 276000, P.R. China.

出版信息

Mol Med Rep. 2019 Mar;19(3):2144-2152. doi: 10.3892/mmr.2019.9870. Epub 2019 Jan 17.

DOI:10.3892/mmr.2019.9870
PMID:30664209
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6390049/
Abstract

The present study aimed to investigate the role and mechanisms of microRNA (miR)‑33b in endometriosis (Ems). Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), MTT assays, flow cytometry, caspase‑3/9 activity assays and western blotting were performed in the present study. Initially, miR‑33b expression in an Ems rat model was investigated by RT‑qPCR and was demonstrated to be upregulated in Ems tissue samples of rats compared with the control group. In addition, miR‑33b upregulation inhibited cell growth and enhanced apoptosis in an Ems model (primary cell cultures) compared with the control group. In addition, miR‑33b up‑regulation reduced Wnt/β‑catenin signaling pathway and suppressed zinc finger E‑box‑binding homeobox 1 (ZEB1) protein expression in the in vitro Ems model (primary cell cultures) compared with the control group. Furthermore, small interfering‑ZEB1 ameliorated the effects of miR‑33b downregulation on Ems cell growth in the in vitro Ems model. Additionally, a Wnt agonist reduced the effects of miR‑33b upregulation on Ems cell growth in the in vitro Ems model. In conclusion, the present study demonstrated that upregulation of miR‑33b may promote Ems through Wnt/β‑catenin by ZEB1 expression.

摘要

本研究旨在探讨微小 RNA（miR）-33b 在子宫内膜异位症（Ems）中的作用和机制。本研究中进行了逆转录-定量聚合酶链反应（RT-qPCR）、MTT 测定、流式细胞术、半胱天冬酶-3/9 活性测定和 Western blot 分析。首先，通过 RT-qPCR 检测 miR-33b 在 Ems 大鼠模型中的表达，结果表明与对照组相比，大鼠 Ems 组织样本中 miR-33b 表达上调。此外，与对照组相比，miR-33b 上调抑制了 Ems 模型（原代细胞培养物）中的细胞生长并增强了细胞凋亡。此外，与对照组相比，miR-33b 上调减少了体外 Ems 模型（原代细胞培养物）中的 Wnt/β-catenin 信号通路，并抑制了锌指 E-框结合同源盒 1（ZEB1）蛋白的表达。此外，小干扰-ZEB1 改善了 miR-33b 下调对体外 Ems 模型中 Ems 细胞生长的影响。此外，Wnt 激动剂降低了 miR-33b 上调对体外 Ems 模型中 Ems 细胞生长的影响。综上所述，本研究表明，miR-33b 的上调可能通过 ZEB1 表达促进 Wnt/β-catenin 促进 Ems。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/8ff068418cfc/MMR-19-03-2144-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/5e646aa9079f/MMR-19-03-2144-g00.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/8ff068418cfc/MMR-19-03-2144-g06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/5e646aa9079f/MMR-19-03-2144-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/770a11096149/MMR-19-03-2144-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/441663f26f93/MMR-19-03-2144-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/c5f7664f1258/MMR-19-03-2144-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/460b39d21259/MMR-19-03-2144-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/633a41c29d11/MMR-19-03-2144-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f1b/6390049/8ff068418cfc/MMR-19-03-2144-g06.jpg

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