1 Department of Environmental Biotechnology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Olsztyn 10-709, Poland.
2 Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Olsztyn 10-713, Poland.
Exp Biol Med (Maywood). 2019 Jan;244(1):52-63. doi: 10.1177/1535370218824573. Epub 2019 Jan 21.
The delivery of short snippets of RNA, such as synthetic miRNA agents, is an essential step for achieving RNA-mediated knockdown, which has not been studied in sufficient detail in fish. Our results indicate that a MiR92b-3p mimic may be effectively delivered via intraperitoneal injection to the spleen and the liver of whitefish, and that it likely achieves functionality without causing any apparent toxic effects in the challenged animals. We report the novel finding that the MiR92b-3p mimic reduced the in vivo liver mRNA expression levels of its putative pro-apoptotic targets (p53, cdkn1a, and pcna), and important metabolic genes, e.g. cdo1. This shows that this methodology of MiR92b-3p mimic transfection in vivo may be a useful tool for studies that investigate the molecular pathways that confer pro-proliferative and anti-apoptotic phenotypes or those that regulate intracellular metabolism in fish and other vertebrates.
短片段 RNA（如合成 miRNA 试剂）的递呈是实现 RNA 介导的基因敲低的关键步骤，但在鱼类中尚未对此进行充分研究。我们的结果表明，miR92b-3p 模拟物可通过腹腔注射有效递送至白鲑的脾脏和肝脏，并且在接受挑战的动物中可能不会产生任何明显的毒性作用而发挥功能。我们报告了一个新发现，即 miR92b-3p 模拟物降低了体内肝脏 mRNA 表达水平其潜在的促凋亡靶标（p53、cdkn1a 和 pcna）和重要的代谢基因，例如 cdo1。这表明，这种体内 miR92b-3p 模拟物转染的方法可能是研究赋予促增殖和抗凋亡表型或调节鱼类和其他脊椎动物细胞内代谢的分子途径的有用工具。
