Goc A, Wegleński P
Department of Plant Cytology and Genetics, Copernicus University, Toruń, Poland.
Curr Genet. 1988 Nov;14(5):425-9. doi: 10.1007/BF00521264.
We have constructed a series of deletion plasmids which contain the Aspergillus nidulans argB gene for ornithine carbamoyltransferase (OTC). These deletions comprise the 5' upstream sequence of the argB gene. The pro- arg- strain of A. nidulans was transformed with the above plasmids. Several arg+ transformants of integration types I and II, obtained using each of the deletion plasmids, were studied, and their ability to de-repress OTC level by proline starvation was compared. It was concluded that nucleotides located between -150 and -50 bp upstream of the argB gene are significant for its cross-pathway regulation. This regulatory region contains three copies of the TGACTC hexanucleotide which is a cis-acting regulatory sequence of general amino acid control in yeast.
我们构建了一系列缺失质粒，这些质粒包含构巢曲霉鸟氨酸氨甲酰基转移酶（OTC）的argB基因。这些缺失包括argB基因的5'上游序列。用上述质粒转化构巢曲霉的pro-arg-菌株。研究了使用每种缺失质粒获得的几种整合类型I和II的arg+转化体，并比较了它们通过脯氨酸饥饿去抑制OTC水平的能力。得出的结论是，位于argB基因上游-150至-50 bp之间的核苷酸对其交叉途径调节具有重要意义。该调节区域包含三个TGACTC六核苷酸拷贝，这是酵母中一般氨基酸控制的顺式作用调节序列。
