Department of Environmental Engineering, Konkuk University, Seoul 05029, Korea.
Department of Environment and Energy, Sejong University, Seoul 05006, Korea.
J Environ Sci (China). 2019 Apr;78:247-256. doi: 10.1016/j.jes.2018.10.003. Epub 2018 Oct 26.
A monitoring method of biofouling in reverse osmosis (RO) system was proposed based on the fluorescent signal of resorufin, which is reduced by nicotinamide adenine dinucleotide released from viable cells during aerobic respiration. The fluorescent signal of resorufin reduced by planktonic cells and microorganisms of biofilm showed linearity, indicating its feasibility to monitor biofouling in a RO system. For the application of the method to the lab-scale RO system, the injection concentration of resazurin and the injection flow rate were optimized. Biofilm on RO membranes continuously operated in a lab-scale RO system was estimated by resorufin fluorescence under optimized detection condition. As a result, resorufin fluorescence on RO membrane showed a significant increase in which the permeability of RO system decreased by 30.48%. Moreover, it represented the development of biofilm as much as conventional biofilm parameters such as adenosine triphosphate, extracellular polymeric substances, and biofilm thickness. The proposed method could be used as a sensitive and low-cost technology to monitor biofouling without autopsy of membranes.
基于生物膜有氧呼吸过程中释放的烟酰胺腺嘌呤二核苷酸还原(resorufin)的荧光信号，提出了一种反渗透(RO)系统生物垢监测方法。浮游细胞和生物膜微生物还原的(resorufin)荧光信号呈线性关系，表明该方法在 RO 系统中监测生物垢的可行性。为了将该方法应用于实验室规模的 RO 系统，优化了 resazurin 的注射浓度和注射流速。在优化的检测条件下，用 resorufin 荧光法估算了在实验室规模的 RO 系统中连续运行的 RO 膜上的生物膜。结果表明，RO 膜上的 resorufin 荧光显著增加，RO 系统的渗透率降低了 30.48%。此外，它与传统的生物膜参数（如三磷酸腺苷、胞外聚合物和生物膜厚度）一样，能够代表生物膜的发展。该方法可以作为一种灵敏、低成本的技术，在不进行膜解剖的情况下监测生物垢。
