Department of Dermatology, China-Japan Union Hospital of Jilin University, Changchun, Jilin, P. R. China; Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, P. R. China.
Institute of Military Veterinary Medicine, Academy of Military Medical Science, Changchun, P. R. China; Changchun University of Chinese Medicine, Changchun, P. R. China.
Urol Oncol. 2019 Jun;37(6):352.e1-352.e18. doi: 10.1016/j.urolonc.2018.12.012. Epub 2019 Jan 18.
Apoptin can specifically kill cancer cells but has no toxicity to normal cells. Human telomerase reverse transcriptase (hTERT) acts as a tumor-specific promoter, triggering certain genes to replicate or express only in tumor cells, conferring specific replication and killing abilities. This study aimed to investigate the anticancer potential of the recombinant adenovirus Ad-apoptin-hTERTp-E1a (Ad-VT) in prostate cancer.
The pGL4.51 plasmid was used to transfect PC-3 cells to construct tumor cells stably expressing luciferase (PC-3-luc). Crystal violet staining and MTS assays determined the ability of Ad-VT to inhibit cell proliferation. Ad-VT-induced apoptosis of PC-3-luc cells was detected using Hoechst, Annexin V, JC-1 staining, and caspases activity analysis. PC-3-luc cells invasion and migration were detected using cell-scratch and Transwell assays. In vivo tumor inhibition was detected using imaging techniques.
Crystal violet staining and MTS results showed that the proliferation ability of PC-3-luc cells decreased significantly. Hoechst, JC-1, and Annexin V experiments demonstrated that Ad-VT mainly induced apoptosis to inhibit PC-3-luc cell proliferation. Ad-VT could significantly inhibit the migration and invasion of PC-3-luc cells over a short period of time. In vivo, Ad-VT could effectively inhibit tumor growth and prolong survival of the mice.
The recombinant adenovirus, comprising the apoptin protein and the hTERTp promoter, was able to inhibit the growth of prostate cancer PC-3 cells and promote their apoptosis.
凋亡素可以特异性地杀死癌细胞，而对正常细胞没有毒性。人端粒酶逆转录酶（hTERT）作为一种肿瘤特异性启动子，仅在肿瘤细胞中触发某些基因的复制或表达，赋予其特异性的复制和杀伤能力。本研究旨在探讨重组腺病毒 Ad-apoptin-hTERTp-E1a（Ad-VT）在前列腺癌中的抗癌潜力。
使用 pGL4.51 质粒转染 PC-3 细胞构建稳定表达荧光素酶的肿瘤细胞（PC-3-luc）。结晶紫染色和 MTS 测定评估 Ad-VT 抑制细胞增殖的能力。采用 Hoechst、Annexin V、JC-1 染色和 caspase 活性分析检测 Ad-VT 诱导 PC-3-luc 细胞凋亡的能力。通过细胞划痕和 Transwell 实验检测 PC-3-luc 细胞的侵袭和迁移。采用成像技术检测体内肿瘤抑制情况。
结晶紫染色和 MTS 结果显示，PC-3-luc 细胞的增殖能力显著下降。Hoechst、JC-1 和 Annexin V 实验表明，Ad-VT 主要通过诱导细胞凋亡来抑制 PC-3-luc 细胞的增殖。Ad-VT 可在短时间内显著抑制 PC-3-luc 细胞的迁移和侵袭。体内实验中，Ad-VT 可有效抑制肿瘤生长并延长小鼠的生存时间。
包含凋亡素蛋白和 hTERTp 启动子的重组腺病毒能够抑制前列腺癌细胞 PC-3 的生长并促进其凋亡。
