Granström M, Wahren B, Gronowicz E, Möller G
Scand J Immunol. 1978 Apr;7(4):277-84. doi: 10.1111/j.1365-3083.1978.tb00455.x.
The effect of dextran-sulphate (DS), a polyclonal activator known to stimulate immature murine B-cells, was assayed in a culture system allowing the growth of myeloid cells. It was known that DS induced the production of a myeloid colony stimulating factor (CSF) by cells from both spleen and bone marrow. Nylonwool purified mouse spleen cells, enriched for T cells, showed a dimished CSF production in response to DS, while CSF production in response to Con A was increased. Furthermore, DS induced CSF in both spleen and bone marrow cells from nude mice. Removal of macrophages did not affect CSF production. The CSF induced was non-dialysable and no small molecular weight or lipoprotein inhibitors could be demonstrated. The results suggest that DS activates cells other than T cells or macrophages (possibly B cells or null cells) to produce a myeloid stem cell stimulating substance. These results indicate that interactions between lymphoid and myeloid cells can take place during differentiation.
已知葡聚糖硫酸酯（DS）是一种能刺激未成熟鼠B细胞的多克隆激活剂，在一个允许髓样细胞生长的培养系统中对其作用进行了测定。已知DS可诱导脾脏和骨髓细胞产生一种髓样集落刺激因子（CSF）。经尼龙毛纯化、富含T细胞的小鼠脾细胞，对DS刺激的CSF产生减少，而对刀豆蛋白A刺激的CSF产生增加。此外，DS可诱导裸鼠脾脏和骨髓细胞产生CSF。去除巨噬细胞不影响CSF的产生。所诱导的CSF不可透析，且未发现小分子或脂蛋白抑制剂。结果表明，DS激活T细胞或巨噬细胞以外的细胞（可能是B细胞或裸细胞）产生一种髓样干细胞刺激物质。这些结果表明，淋巴细胞和髓样细胞之间的相互作用可在分化过程中发生。
