Gronowicz E, Biberfeld P, Wahren B, Coutinho A
Scand J Immunol. 1976;5(5):573-82. doi: 10.1111/j.1365-3083.1976.tb00312.x.
The cell type participating in the mitotic response to the polyclonal B-cell activator (PBA) dextran-sulfate (DS) was investigated. Cells from murine fetal liver, adult bone marrow, and spleen were studied; only a limited number of all cells present in each organ responded to DS. Morphological studies of the activated cells showed the major population of activated cells in spleen to have the appearance of lymphoblasts. In bone marrow, several classes of hematopoietic cells were mitotically active, including mononuclear cells (lymphoblasts and monocytes), megakaryocytes, and myeloblasts. In these cultures, however, it was not possible to differentiate between DS-activated and spontaneously proliferating cells. Bone marrow and, to some extent, spleen cell cultures activated with DS contained a relative increase in numbers of phagocytic cells, whereas stimulation of spleen cells with lipopolysaccharide did not result in an increased phagocytosis. However, adherent cells were not necessary for activation of DNA synthesis by DS in spleen, and this cell type did not contribute to a measurable degree to the DNA synthetical response. DS cannot be regarded as a general stem cell mitogen for bone marrow cells since it failed to promote colony growth of hematopoietic cells in an in vitro system. However, supernatants from DS-activated spleen and bone marrow cell cultures did stimulate colony growth of murine bone marrow cells, indicating that stem cells of nonlymphoid origin might be indirectly activated by DS. In conclusion, the major cell population activated by DS in spleen is lymphocytes. In bone marrow, other cell types seem to participate in the response as well, but the activation mechanism may be indirect and not primarily the result of DS interaction with these cells.
研究了参与对多克隆B细胞激活剂（PBA）硫酸葡聚糖（DS）有丝分裂反应的细胞类型。对来自小鼠胎肝、成年骨髓和脾脏的细胞进行了研究；每个器官中存在的所有细胞中只有有限数量的细胞对DS有反应。对活化细胞的形态学研究表明，脾脏中活化细胞的主要群体具有淋巴母细胞的外观。在骨髓中，几类造血细胞具有有丝分裂活性，包括单核细胞（淋巴母细胞和单核细胞）、巨核细胞和成髓细胞。然而，在这些培养物中，无法区分DS活化的细胞和自发增殖的细胞。用DS活化的骨髓以及在一定程度上脾脏细胞培养物中吞噬细胞数量相对增加，而用脂多糖刺激脾脏细胞不会导致吞噬作用增加。然而，贴壁细胞对于脾脏中DS激活DNA合成并非必需，并且这种细胞类型对DNA合成反应的贡献程度无法测量。DS不能被视为骨髓细胞的一般干细胞有丝分裂原，因为它在体外系统中未能促进造血细胞的集落生长。然而，DS活化的脾脏和骨髓细胞培养物的上清液确实刺激了小鼠骨髓细胞的集落生长，表明非淋巴样来源的干细胞可能被DS间接激活。总之，脾脏中被DS激活的主要细胞群体是淋巴细胞。在骨髓中，其他细胞类型似乎也参与了反应，但激活机制可能是间接的，并非主要是DS与这些细胞相互作用的结果。
