Johnson G R
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Dev Biol Stand. 1988;69:3-8.
To study the effects of dysregulated granulocyte-macrophage colony stimulating factor (GM-CSF) production, a retroviral vector (MPZen) containing a GM-CSF cDNA has been used to infect murine bone marrow cells prior to their transplantation into lethally-irradiated recipients. Animals transplanted with 1 x 10(6) bone marrow cells 14 to 21 days after transplantation displayed shortness of breath, trembling to the touch, and dragging of the hind limbs. These animals were shown to have increased peripheral blood neutrophil counts (x 200), decreased marrow cellularity (but with increased neutrophil and macrophage frequency) and increased spleen cellularity (with up to a 40-fold increase in neutrophil, macrophage and eosinophil frequency). Examination of sections of tissues from infected mice showed massive infiltration of neutrophils and macrophages in muscle, lungs, liver, and variable infiltration of these cells in lymphoid tissues and the eye. Haemopoietic tissues produced high levels of GM-CSF (10(5) Units/10(6) cells) and the serum contained up to 5.5 x 10(5) Units of GM-CSF per ml.
为研究粒细胞巨噬细胞集落刺激因子（GM-CSF）产生失调的影响，一种含有GM-CSF cDNA的逆转录病毒载体（MPZen）已被用于在将小鼠骨髓细胞移植到接受致死剂量照射的受体之前感染这些细胞。移植后14至21天接受1×10⁶个骨髓细胞移植的动物出现呼吸急促、触碰时颤抖以及后肢拖地的症状。这些动物外周血中性粒细胞计数增加（增至200倍），骨髓细胞密度降低（但中性粒细胞和巨噬细胞频率增加），脾脏细胞密度增加（中性粒细胞、巨噬细胞和嗜酸性粒细胞频率增加多达40倍）。对感染小鼠组织切片的检查显示，肌肉、肺、肝中有大量中性粒细胞和巨噬细胞浸润，在淋巴组织和眼中有这些细胞的不同程度浸润。造血组织产生高水平的GM-CSF（10⁵单位/10⁶个细胞），血清中每毫升含有高达5.5×10⁵单位的GM-CSF。
