Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB, T6G 2P5, Canada.
Institute of Dairy Science, MoE Key Laboratory of Molecular Animal Nutrition, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, P.R. China.
Sci Data. 2019 Jan 22;6:180301. doi: 10.1038/sdata.2018.301.
With the help of the bacteria in the rumen, ruminants can effectively convert human inedible plant fiber to edible food (meat and milk). However, the understanding of rumen bacteriome in dairy cows is still limited, especially in a large population under the same diet, breed, and milking period. Here we described the sequencing data of 16S rRNA gene of rumen bacteriome from 334 mid-lactation Holstein dairy cows generated using the Illumina HiSeq 2500 (PE250) platform. A total of 24,030,828 raw reads with an average of 71,946 ± 13,450 sequences per sample were obtained. The top ten genera with highest relative abundance accounted for 60.65% of total bacterial sequences. We observed 4,460 overall operational taxonomic units (1,827 ± 94 per sample) based on a 97% nucleotide sequence identity between reads. Totally 6,082 amplicon sequence variants (672 ± 131 per sample) were identified in 334 samples. The shareable datasets can be re-used by researchers to assess other rumen bacterial-related biological functions in dairy cows towards the improvement of animal production and health.
在瘤胃细菌的帮助下，反刍动物可以有效地将人类不可食用的植物纤维转化为可食用的食物（肉和奶）。然而，人们对奶牛瘤胃微生物组的了解仍然有限，特别是在同一饮食、品种和泌乳期的大量奶牛中。本研究描述了使用 Illumina HiSeq 2500（PE250）平台对 334 头泌乳中期荷斯坦奶牛的瘤胃微生物组 16S rRNA 基因进行测序得到的结果。共获得 24,030,828 条原始reads，平均每个样本有 71,946±13,450 条序列。丰度最高的前 10 个属占总细菌序列的 60.65%。基于reads 之间 97%的核苷酸序列同一性，我们观察到 4,460 个总体分类操作单元（每个样本 1,827±94 个）。在 334 个样本中共鉴定出 6,082 个扩增子序列变异体（每个样本 672±131 个）。这些可共享的数据集可供研究人员重新用于评估奶牛瘤胃细菌相关的其他生物学功能，以改善动物生产和健康。
