齿叶黄皮素（Clausena excavata Burm.f.）抗溃疡活性对乙醇诱导的大鼠胃溃疡的作用：可能与粘液和抗氧化作用有关。
Anti-ulcerogenic activity of dentatin from clausena excavata Burm.f. against ethanol-induced gastric ulcer in rats: Possible role of mucus and anti-oxidant effect.
机构信息
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
出版信息
Phytomedicine. 2019 Mar 1;55:31-39. doi: 10.1016/j.phymed.2018.06.036. Epub 2018 Aug 3.
BACKGROUND
Clausena excavata Burm.f. (Rutaceae) has been used for the treatment of stomach disorders including peptic ulcer.
PURPOSE
In this study, we aimed to investigate dentatin isolated from C. excavata Burm.f., for anti-ulcer activity against ethanol ulcer model in rats.
METHODS
Gastric acid output, ulcer index, serum profile, histological evaluation using Hematoxylin and eosin (HE), periodic acid Schiff base stainings and immunohistochemical localization for heat shock proteins 70 (HSP70) were all investigated. Possible involvement of reduced glutathione (GSH), lipid peroxidation, prostaglandin E (PGE), superoxide dismutase (SOD) enzymes, radical scavenging, and anti-Helicobacter pylori activity were investigated.
RESULTS
Dentatin showed anti-secretory activity against the pylorus ligature model and protected the gastric mucosa from ethanol ulceration, as revealed by the improved macroscopic and histological appearance. Dentatin significantly increased the gastric homogenate content of PGE GSH and SOD. Dentatin inhibited the lipid peroxidation as revealed by the reduced gastric content of malondialdehyde (MDA). Moreover, dentatin up-regulated HSP70 expression. However, dentatin showed insignificant anti-H. pylori activity.
CONCLUSION
Dentatin possesses gastro-protective activity, which could be attributed to the anti-secretory, mucus production, anti-oxidant, and HSP70 activities.
背景
吴茱萸（芸香科）已被用于治疗包括胃溃疡在内的胃部疾病。
目的
本研究旨在研究从吴茱萸中分离出的齿叶吴茱萸堿，以研究其对乙醇溃疡模型大鼠的抗溃疡活性。
方法
研究了胃酸分泌、溃疡指数、血清谱、苏木精和伊红（HE）、过碘酸希夫基底染色的组织学评价以及热休克蛋白 70（HSP70）的免疫组织化学定位。还研究了还原型谷胱甘肽（GSH）、脂质过氧化、前列腺素 E（PGE）、超氧化物歧化酶（SOD）酶、自由基清除和抗幽门螺杆菌活性的可能参与。
结果
齿叶吴茱萸堿对幽门结扎模型具有抗分泌作用，并通过改善宏观和组织学外观保护胃黏膜免受乙醇溃疡，齿叶吴茱萸堿显著增加了胃匀浆中 PGE、GSH 和 SOD 的含量。齿叶吴茱萸堿抑制了脂质过氧化，表现为胃内容物丙二醛（MDA）减少。此外，齿叶吴茱萸堿上调了 HSP70 的表达。然而，齿叶吴茱萸堿显示出对 H. pylori 的活性不显著。
结论
齿叶吴茱萸堿具有胃保护活性，这可能归因于其抗分泌、粘液产生、抗氧化和 HSP70 活性。
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