School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China; State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China; College of Environmental Science and Engineering, Guangdong Petrochemical College, Maoming, 525000, China.
School of Environment and Energy, South China University of Technology, Guangzhou, 510006, China; State Key Laboratory of Lake Science and Environment, Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing, 210008, China; State Key Laboratory of Pollution Control and Resource Reuse, Nanjing, 210093, China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangzhou, 510640, China; College of Environmental Science and Engineering, Guangdong Petrochemical College, Maoming, 525000, China.
Environ Pollut. 2019 Apr;247:165-171. doi: 10.1016/j.envpol.2018.12.086. Epub 2019 Jan 9.
The frequent outbreaks of cyanobacteria bloom are often accompanied by the generation and release of reduced phosphorus species (e.g., phosphine), which raises interesting questions regarding their potential algae-related effects. To clarify the physiological and biochemical responses of cyanobacteria to phosphine, Microcystis aeruginosa was treated with different concentrations of phosphine. Net photosynthetic rate, total antioxidant capacity (T-AOC), catalase (CAT) activity, and the concentrations of chlorophyll a, carotenoid and total protein were investigated and scanning electron microscopy (SEM) was conducted to elucidate the physiological and biochemical responses of M. aeruginosa to phosphine. The results showed that phosphine was beneficial to the growth of algal cells after M. aeruginosa acclimatized to the treatment of phosphine, and treatment with 2.48 × 10 mg/L phosphine had a greater positive effect on the growth and reproduction of M. aeruginosa than 7.51 × 10 mg/L phosphine, in which most algal cells were smooth and flat on day 16. Treatment with the high concentration of phosphine (7.51 × 10 mg/L) for 16 d reduced T-AOC, CAT activity, net photosynthetic rate, and the concentrations of chlorophyll a, carotenoid and total protein of M. aeruginosa to the minimums, resulting in the lysis and death of M. aeruginosa cells, which indicates phosphine has a toxic effect on the growth of algal cells. However, the high concentration of phosphine (7.51 × 10 mg/L) had a greater positive effect on the growth of M. aeruginosa cells than the lower two (7.51 × 10 mg/L and 2.48 × 10 mg/L) from 3 d to 12 d. Our findings provide insight into how phosphine potentially affects the growth of M. aeruginosa cells and the important roles of elevated phosphine on the outbreak of cyanobacteria bloom.
蓝藻水华的频繁暴发常伴随着还原性磷物种（如磷化氢）的产生和释放，这引发了关于其潜在藻类相关效应的有趣问题。为了阐明蓝藻对磷化氢的生理生化响应，用不同浓度的磷化氢处理铜绿微囊藻。研究了净光合速率、总抗氧化能力（T-AOC）、过氧化氢酶（CAT）活性以及叶绿素 a、类胡萝卜素和总蛋白的浓度，并通过扫描电子显微镜（SEM）阐明了铜绿微囊藻对磷化氢的生理生化响应。结果表明，在铜绿微囊藻适应磷化氢处理后，磷化氢有利于藻细胞的生长，与 7.51×10 mg/L 磷化氢相比，2.48×10 mg/L 磷化氢对铜绿微囊藻的生长和繁殖有更大的积极影响，其中大多数藻细胞在第 16 天变得光滑和平坦。用高浓度（7.51×10 mg/L）磷化氢处理 16 d 可使铜绿微囊藻的 T-AOC、CAT 活性、净光合速率以及叶绿素 a、类胡萝卜素和总蛋白的浓度降至最低，导致铜绿微囊藻细胞裂解和死亡，表明磷化氢对藻细胞的生长具有毒性作用。然而，高浓度（7.51×10 mg/L）磷化氢对铜绿微囊藻细胞的生长比低浓度（7.51×10 mg/L 和 2.48×10 mg/L）从第 3 天到第 12 天更有积极影响。我们的研究结果为磷化氢如何影响铜绿微囊藻细胞的生长提供了深入的了解，并为高浓度磷化氢在蓝藻水华暴发中的重要作用提供了重要的启示。
