Wang Xiaoxiong, Jiang Chenchun, Szeto Yim-Tong, Li Ho-Kin, Yam Kwei-Lam, Wang Xiaojun
College of Environment and Energy, South China University of Technology, Guangzhou, 510006, China.
School of Civil and Environmental Engineering, Shenzhen Polytechnic, Shenzhen, 518055, China.
Environ Sci Pollut Res Int. 2016 May;23(9):8731-40. doi: 10.1007/s11356-016-6119-1. Epub 2016 Jan 23.
Harmful cyanobacteria bloom contributes to economic loss as well as the threat to human health. Agricultural waste products, particularly straw, have been used to control bloom while arbor plant is the potential candidate for limiting antialgal activity. This study investigated the use of Dracontomelon duperreanum defoliation extract (DDDE) to inhibit the activity of Microcystis aeruginosa. The primary goal of the research was to explore the solution to control cyanobacterial bloom. The photosynthetic activity, cell morphology, membrane integrity, and esterase activity of M. aeruginosa were determined using phytoplankton analyzer pulse amplitude modulation (Phyto-PAM) and flow cytometry before and after exposure to DDDE. The inhibitory rate of M. aeruginosa was about 99.6 % on day 15 when exposed to 2.0 g L(-1). A reduction of chlorophyll a (Chl-a) activity and changes in cell membrane suggested the algistatic property of DDDE. Inhibition of photosynthetic activity was reflected by changing mean Chl-a fluorescence intensity (MFI) which was about 52.5 % on day 15 when exposed to 2.0 g L(-1) DDDE as well as relative electron transport rates (rETRs) of algal cell. These changes might contribute to the suppression of M. aeruginosa. Algal cell exposed to DDDE may lead to cell volume reduction or slow growth. This resulted in a decreased proportion of normal or swollen granular cells after DDDE treatment.
有害蓝藻水华不仅会造成经济损失，还会对人类健康构成威胁。农业废弃物，尤其是秸秆，已被用于控制水华，而乔木植物是限制藻类活性的潜在候选者。本研究调查了人面子落叶提取物（DDDE）对铜绿微囊藻活性的抑制作用。该研究的主要目标是探索控制蓝藻水华的解决方案。在暴露于DDDE之前和之后，使用浮游植物分析仪脉冲幅度调制（Phyto-PAM）和流式细胞仪测定铜绿微囊藻的光合活性、细胞形态、膜完整性和酯酶活性。当暴露于2.0 g L⁻¹时，第15天铜绿微囊藻的抑制率约为99.6%。叶绿素a（Chl-a）活性的降低和细胞膜的变化表明了DDDE的抑藻特性。光合活性的抑制通过平均Chl-a荧光强度（MFI）的变化来反映，当暴露于2.0 g L⁻¹ DDDE时，第15天MFI约为52.5%，以及藻类细胞的相对电子传递速率（rETR）。这些变化可能有助于抑制铜绿微囊藻。暴露于DDDE的藻类细胞可能导致细胞体积减小或生长缓慢。这导致DDDE处理后正常或肿胀颗粒细胞的比例降低。
